Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Mar 12;29(1):167.
doi: 10.1186/s40001-024-01768-w.

Effects of total gonadotropin dose on embryo quality and clinical outcomes with AMH stratification in IVF cycles: a retrospective analysis of 12,588 patients

Xiaoxue Shen  1   2   3   4 , Yuhan Guo  1   2   3   4 , Yan Liu  1   2   3   4 , Wenyan Song  1   2   3   4 , Gang Li  1   2   3   4 , Haixia Jin  5   6   7   8
Affiliations

Effects of total gonadotropin dose on embryo quality and clinical outcomes with AMH stratification in IVF cycles: a retrospective analysis of 12,588 patients

Xiaoxue Shen et al. Eur J Med Res. .

Abstract

Background: Recent studies about the effect of gonadotropin (Gn) dose on the clinical outcomes of IVF are still controversial, and no studies have analyzed the relationship between Gn dose and embryo quality. Since AMH is a strong predictor of oocyte quality, we aim to evaluate the relationship between total Gn dose and embryo quality and clinical outcomes at different AMH levels in IVF cycles.

Methods: A total of 12,588 patients were enrolled in the retrospective study. The included cycles were categorized by serum AMH levels (AMH ≤ 1 ng/ml, 1 ng/ml < AMH ≤ 3 ng/ml, 3 ng/ml < AMH ≤ 5 ng/ml, AMH > 5 ng/ml), total Gn dosage (< 1875 IU, 1875-3750 IU and ≥ 3750 IU) and female age (< 35 years and 35-42 years). The embryo quality and clinical outcomes were the measure outcomes.

Results: The top-day3 embryos rate decreased with the increase of total Gn dose in nearly all age and AMH subgroups, but this trend was not obvious in the AMH > 5 ng/ml group and AMH ≤ 1 ng/ml group. The blastocyst formation rate and high-quality blastulation rate had a negative relationship with Gn does for women aged < 35 years in the AMH ≤ 5 ng/ml groups, except for the AMH > 5 ng/ml group (P < 0.001). However, when women were 35-42 years old, regardless of AMH levels, the blastocyst formation rate and high-quality blastulation rate decreased as Gn dose increased. Clinical outcomes (implantation rate, clinical pregnancy rate and live birth rate) decreased with the increase of Gn dose in all ages and AMH stratifications.

Conclusions: The total dose of Gn may have different effects on embryo quality at different serum AMH levels, and the negative effects of total dose of Gn on clinical outcomes may be realized by impairing both embryo quality and endometrium.

Keywords: Anti-Müllerian hormone; Clinical outcomes; Embryo quality; Gonadotropin; In vitro fertilization.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Flowchart showing data selection process and cycle distribution
Fig. 2
Fig. 2
The embryo quality of different total Gn dosage groups with AMH stratification. A1, B1, C1: female age < 35 years; A2, B2, C2: female aged 35–42 years. AMH anti-Müllerian hormone, Gn gonadotropin. Group A: < 1875 IU, Group B: 1875–3750 IU, Group C: ≥ 3750 IU. *P < 0.05, **P < 0.01, ***P < 0.001
Fig. 3
Fig. 3
The clinical outcome of different total Gn dosage groups with AMH stratification. The IVF-ET cycles of 2 embryos at the cleavage stage with endometrial thickness ≥ 7 mm on ET day were included. D1, E1, F1: female age < 35 years; D2, E2, F2: female aged 35–42 years. AMH anti-Müllerian hormone, Gn gonadotropin. Group A: < 1875 IU, Group B: 1875–3750 IU, Group C: ≥ 3750 IU. *P < 0.05, **P < 0.01, ***P < 0.001
See this image and copyright information in PMC

References

    1. Macklon NS, Stouffer RL, Giudice LC, Fauser BCJM. The science behind 25 Years of ovarian stimulation for in vitro fertilization. Endocr Rev. 2006;27:170–207. doi: 10.1210/er.2005-0015. - DOI - PubMed
    1. Sunkara SK, Rittenberg V, Raine-Fenning N, Bhattacharya S, Zamora J, Coomarasamy A. Association between the number of eggs and live birth in IVF treatment: an analysis of 400 135 treatment cycles. Hum Reprod. 2011;26:1768–1774. doi: 10.1093/humrep/der106. - DOI - PubMed
    1. Steward RG, Lan L, Shah AA, Yeh JS, Price TM, Goldfarb JM, et al. Oocyte number as a predictor for ovarian hyperstimulation syndrome and live birth: an analysis of 256,381 in vitro fertilization cycles. Fertil Steril. 2014;101:967–973. doi: 10.1016/j.fertnstert.2013.12.026. - DOI - PubMed
    1. Fatemi HM, Doody K, Griesinger G, Witjes H, Mannaerts B. High ovarian response does not jeopardize ongoing pregnancy rates and increases cumulative pregnancy rates in a GnRH-antagonist protocol. Hum Reprod. 2013;28:442–452. doi: 10.1093/humrep/des389. - DOI - PubMed
    1. Ishihara O, Klein BM, Arce J-C, Kuramoto T, Yokota Y, Mukaida T, et al. Randomized, assessor-blind, antimüllerian hormone–stratified, dose-response trial in Japanese in vitro fertilization/intracytoplasmic sperm injection patients undergoing controlled ovarian stimulation with follitropin delta. Fertil Steril. 2021;115:1478–1486. doi: 10.1016/j.fertnstert.2020.10.059. - DOI - PubMed

LinkOut - more resources