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. 2024 Feb 27:15:1334909.
doi: 10.3389/fpls.2024.1334909. eCollection 2024.

Autofluorescence-spectral imaging for rapid and invasive characterization of soybean for pre-germination anaerobic stress tolerance

Ambika Rajendran  1 , Ayyagari Ramlal  1   2 , Subham Sarkar  3 , Sarit S Agasti  3 , K Rajarajan  4 , S K Lal  1 , Dhandapani Raju  5 , Sreeramanan Subramaniam  2   6   7
Affiliations

Autofluorescence-spectral imaging for rapid and invasive characterization of soybean for pre-germination anaerobic stress tolerance

Ambika Rajendran et al. Front Plant Sci. .

Abstract

The autofluorescence-spectral imaging (ASI) technique is based on the light-emitting ability of natural fluorophores. Soybean genotypes showing contrasting tolerance to pre-germination anaerobic stress can be characterized using the photon absorption and fluorescence emission of natural fluorophores occurring in seed coats. In this study, tolerant seeds were efficiently distinguished from susceptible genotypes at 405 nm and 638 nm excitation wavelengths. ASI approach can be employed as a new marker for the detection of photon-emitting compounds in the tolerant and susceptible soybean seed coats. Furthermore, the accuracy of rapid characterization of genotypes using this technique can provide novel insights into soybean breeding.

Keywords: autofluorescence; imaging; soybean; spectral markers; susceptible; tolerant.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Autofluorescence intensity profile in genotype tolerant (A–C), susceptible (D–F), moderately tolerant (G–J) seed coats from soybean at four channels (405, 488, 552 and 638 nm) (Bar = 200 µm). (X axis, emission intensity; Y axis, line of projection in µm).
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