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. 2023 Jan-Feb:12391:1239106.
doi: 10.1117/12.2650815. Epub 2023 Mar 16.

An automatic whole-slide hyperspectral imaging microscope

Minh Ha Tran  1   2 , Ofelia Gomez  1   2 , Baowei Fei  1   2   3
Affiliations

An automatic whole-slide hyperspectral imaging microscope

Minh Ha Tran et al. Proc SPIE Int Soc Opt Eng. 2023 Jan-Feb.

Abstract

Whole slide imaging (WSI) is a common step used in histopathology to quickly digitize stained histological slides. Digital whole-slide images not only improve the efficiency of labeling but also open the door for computer-aided diagnosis, specifically machine learning-based methods. Hyperspectral imaging (HSI) is an imaging modality that captures data in various wavelengths, some beyond the range of visible lights. In this study, we developed and implemented an automated microscopy system that can acquire hyperspectral whole slide images (HWSI). The system is robust since it consists of parts that can be swapped and bought from different manufacturers. We used the automated system and built a database of 49 HWSI of thyroid cancer. The automatic whole-slide hyperspectral imaging microscope can have many potential applications in biological and medical areas.

Keywords: Hyperspectral imaging; automation; database; deep learning; whole-slide imaging.

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Figures

Figure 1.
Figure 1.
(a) Our customized, automatic hyperspectral imaging microscopic system. (b) The flowchart for the whole-slide hyperspectral image acquisition.
Figure 2.
Figure 2.
Image captured using the Snapscan system at the wavelength of 614 nm, showing the clear features of the elements in Group 7.
Figure 3.
Figure 3.
A selection of two slides captured with a conventional RGB whole slide imaging scanner (top row), compared with the same slide captured with our HWSI imager and then synthesized to produce RGB images (bottom row). The labels (normal, cancer) were determined by a pathologist.
Figure 4.
Figure 4.
A section of the whole slide image from the RGB and the WHSI at five different wavelengths.
See this image and copyright information in PMC

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