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. 1985 Jul;51(1):41-7.
doi: 10.1007/BF00320599.

Transferrin in isolated cells from rat duodenum and jejunum

K Osterloh, K Schümann, C Ehtechami, W Forth

Transferrin in isolated cells from rat duodenum and jejunum

K Osterloh et al. Blut. 1985 Jul.

Abstract

Mucosal transferrin was determined as transferrin-like immunoreactivity (TLIR) by means of a 2-site immunoradiometric assay (IRMA). Scraped-off mucosal tissue as well as isolated mucosal cells from the duodenum and jejunum of normal and iron-deficient rats before and after a washing procedure were examined. In iron-deficient rats there was about twice as much TLIR in scraped-off mucosal tissue as in the untreated animals. In the duodenum and jejunum of normal and iron-deficient rats, TLIR contents of the isolated cells in the magnitude of 320-510 ng/mg dry weight were found. Washing isolated cells three times in ice-cold Hank's solution resulted in a nearly tenfold decrease of TLIR content in all groups. In contrast the cells' RNA content remained unchanged.

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