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. 1985 Feb;4(2):309-16.
doi: 10.1002/j.1460-2075.1985.tb03630.x.

Evidence for coiled-coil alpha-helical regions in the long arm of laminin

M Paulsson, R Deutzmann, R Timpl, D Dalzoppo, E Odermatt, J Engel

Evidence for coiled-coil alpha-helical regions in the long arm of laminin

M Paulsson et al. EMBO J. 1985 Feb.

Abstract

Three new laminin fragments, E8, E9 and 25K with mol. wt. 50 000-280 000, were prepared from a limited elastase digest of laminin and from tissue extracts. They were similar with respect to their rod-like structure, a high alpha-helix content, the assembly from two chain segments and immunological cross-reactivity. Two of the fragments (E8 and E9) possess in addition globular domains which lack alpha-helices. Chemical, immunological and physical data together with sequence analysis strongly indicate that the alpha-helical segments are assembled in coiled-coil structures which are located in the rod of the long arm of laminin. These data give new insights into the overall structure of the protein.

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