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. 2024 Mar;69(1):922-928.
doi: 10.1007/s11686-024-00815-0. Epub 2024 Mar 15.

Identification of Blastocystis spp. in Urban Rodents of Different Districts in Southwestern Iran: Subtype Distribution and Possible Zoonotic Potential

Saiyad Bastaminejad  1 , Parand Eskandari  2 , Mohammad Reza Mohammadi  3 , Laya Shamsi  4 , Ali Asghari  5 , Saeed Shahabi  6 , Mostafa Omidian  2 , Ali Pouryousef  2 , Bahador Shahriari  7   8
Affiliations

Identification of Blastocystis spp. in Urban Rodents of Different Districts in Southwestern Iran: Subtype Distribution and Possible Zoonotic Potential

Saiyad Bastaminejad et al. Acta Parasitol. 2024 Mar.

Abstract

Purpose: Rodents are one of the most abundant and diverse species of mammals and have recently been identified as carriers of numerous human pathogens. The current study was conducted to assess the prevalence, subtype (STs) distribution, and zoonotic potential of Blastocystis spp. in various species of rodents in Shiraz, southwestern Iran.

Methods: For this aim, a total of 120 fresh fecal samples were collected from Mus musculus (n = 40), Rattus norvegicus (n = 40), and Rattus rattus (n = 40) in various municipality districts of Shiraz (6 out of 10 districts) between February and November 2020. Upon detecting parasites using light microscopy, a DNA fragment of the Blastocystis SSU rDNA gene was amplified using conventional PCR.

Results: By employing direct wet mount examination, 8 out of 120 fecal samples (6.7%; 2 from house mice, 3 from black rats, and 3 from brown rats) tested positive. Similarly, 5% (2/40) of house mice, 7.5% (3/40) of black rats, and 7.5% (3/40) of brown rats tested positive using the molecular method. Phylogenetic analysis revealed that the Blastocystis infecting different rodent species in Shiraz belonged to two potentially zoonotic STs (ST1 and ST4). Accordingly, rodents should not be overlooked as potential reservoirs of zoonotic Blastocystis infections. Different sampled urban districts and their statistical association with reported prevalence rates were analyzed separately.

Conclusion: Overall, the issue of the frequency and ST distribution of Blastocystis in urban rodents of Iran is still open to question and for a proper understanding, wider and more comprehensive studies are needed.

Keywords: Blastocystis spp.; Animals; Iran; Rodents; Subtypes.

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References

    1. Shams M, Shamsi L, Yousefi A, Sadrebazzaz A, Asghari A, Mohammadi-Ghalehbin B, Shahabi S, Hatam G (2022) Current global status, subtype distribution and zoonotic significance of Blastocystis in dogs and cats: a systematic review and meta-analysis. Parasites Vectors 15:225. https://doi.org/10.1186/s13071-022-05351-2 - DOI - PubMed - PMC
    1. Jiménez P, Muñoz M, Ramírez JD (2022) An update on the distribution of Blastocystis subtypes in the Americas. Heliyon 8:e12592 - DOI - PubMed - PMC
    1. Tantrawatpan C, Vaisusuk K, Thanchomnang T, Pilap W, Sankamethawee W, Suksavate W, Chatan W, Bunchom N, Kaewkla O, Stensvold CR (2023) Distribution of Blastocystis subtypes isolated from various animal hosts in Thailand. Res Vet Sci 162:104939. https://doi.org/10.1016/j.rvsc.2023.06.017 - DOI - PubMed
    1. Asghari A, Sadraei J, Pirestani M, Mohammadpour I (2019) First molecular identification and subtype distribution of Blastocystis sp. isolated from hooded crows (Corvus cornix) and pigeons (Columba livia) in Tehran Province, Iran. Comp Immunol Microbiol Infect Dis 62:25–30. https://doi.org/10.1016/j.cimid.2018.11.013 - DOI - PubMed
    1. Chen H, Hao Y, Liu Y, Xu M, Zhang W, Li H, Yang F (2023) The frequency and subtype distribution of Blastocystis sp. in humans and domestic animals in households in Heilongjiang province, China. Acta Trop 240:106844. https://doi.org/10.1016/j.actatropica.2023.106844 - DOI - PubMed

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