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. 1979 Jun;37(6):1173-5.
doi: 10.1128/aem.37.6.1173-1175.1979.

Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin

S Notermans, J Dufrenne, S Kozaki

Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin

S Notermans et al. Appl Environ Microbiol. 1979 Jun.

Abstract

The enzyme-linked immunosorbent assay using the "double-sandwich" technique was utilized to determine Clostridium botulinum type E toxin. With this technique, about 80 mouse intraperitoneal 50% lethal doses of toxin could be detected. Cross-reaction was hardly observed with C. botulinum type A and B toxins. No cross-reaction was observed with culture supernatants of C. botulinum type C or other Clostridium strains. In all probability this was due to the high specificity of the antiserum prepared aginst the toxic component of type E toxin.

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