Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Jun;37(6):1173-5.
doi: 10.1128/aem.37.6.1173-1175.1979.

Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin

S Notermans, J Dufrenne, S Kozaki

Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin

S Notermans et al. Appl Environ Microbiol. 1979 Jun.

Abstract

The enzyme-linked immunosorbent assay using the "double-sandwich" technique was utilized to determine Clostridium botulinum type E toxin. With this technique, about 80 mouse intraperitoneal 50% lethal doses of toxin could be detected. Cross-reaction was hardly observed with C. botulinum type A and B toxins. No cross-reaction was observed with culture supernatants of C. botulinum type C or other Clostridium strains. In all probability this was due to the high specificity of the antiserum prepared aginst the toxic component of type E toxin.

PubMed Disclaimer

References

    1. Jpn J Med Sci Biol. 1978 Feb;31(1):81-5 - PubMed
    1. Bull World Health Organ. 1974;51(1):108-9 - PubMed
    1. J Immunol. 1972 Jul;109(1):129-35 - PubMed
    1. J Histochem Cytochem. 1974 Dec;22(12):1084-91 - PubMed
    1. Arch Biochem Biophys. 1969 Nov;134(2):279-84 - PubMed

Substances

LinkOut - more resources