Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Mar 16;206(4):172.
doi: 10.1007/s00203-024-03885-w.

The usefulness of the MALDI-TOF MS technique in the determination of dairy samples' microbial composition: comparison of the new EXS 2600 system with MALDI Biotyper platform

Grażyna Czeszewska-Rosiak  1   2 , Michał Złoch  3 , Monika Radosińska  2 , Aleksandra Bogumiła Florkiewicz  2 , Andrzej Tretyn  1   2 , Paweł Pomastowski  2
Affiliations

The usefulness of the MALDI-TOF MS technique in the determination of dairy samples' microbial composition: comparison of the new EXS 2600 system with MALDI Biotyper platform

Grażyna Czeszewska-Rosiak et al. Arch Microbiol. .

Abstract

This study compared the EXS 2600 system with the MALDI Biotyper for identifying microorganisms in dairy samples. Of the 196 bacterial isolates from milk, whey, buttermilk, cream, and dairy wastewater, the species and genus consistent identification between two systems showed 74% and 99%, respectively. However, the level of species identification rate exhibited a difference, which was higher in Zybio than in Bruker-76.0% and 66.8%, respectively. Notably, the EXS 2600 system performed better with certain yeast species and H. alvei, while the Biotyper excelled with Pseudomonas bacteria. Unique microbial compositions were found in 85% of dairy samples, with whey and buttermilk having the highest diversity. This research highlights the EXS 2600's potential as a reliable dairy microbial identification tool and underscores the need for a more diverse and comprehensive spectral database, despite the database's focus on clinical applications (as announced).

Keywords: Bacteria; Biotyper; Dairy products; EXS 2600; MALDI; Microorganism identification.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Akimowicz M, Bucka-Kolendo J (2020) MALDI–TOF MS-application in food microbiology. Acta Biochim Pol 67(3):327–332. https://doi.org/10.18388/ABP.2020_5380 - DOI - PubMed
    1. Bizzini A, Jaton K, Romo D, Bille J, Prod’hom, G., Greub, G, (2011) Matrix-assisted laser desorption ionization—time of flight mass spectrometry as an alternative to 16S RRNA gene sequencing for identification of difficult-to-identify bacterial strains. J Clin Microbiol 49(2):693–696. https://doi.org/10.1128/JCM.01463-10 - DOI - PubMed - PMC
    1. Body BA, Beard MA, Slechta ES, Hanson KE, Barker AP, Babady NE, McMillen T, Tang YW, Brown-Elliott BA, Iakhiaeva E, Vasireddy R, Vasireddy S, Smith T, Wallace RJ, Turner S, Curtis L, Butler-Wu S, Rychert J (2018) Evaluation of the Vitek MS v3.0 matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of mycobacterium and nocardia species. J Clin Microbiol. https://doi.org/10.1128/JCM.00237-18 - DOI - PubMed - PMC
    1. Böhme K, Fernández-No IC, Barros-Velázquez J, Gallardo JM, Cañas B, Calo-Mata P (2012) Species identification of food spoilage and pathogenic bacteria by MALDI–TOF mass fingerprinting. Food Qual. https://doi.org/10.5772/33884 - DOI
    1. Brodzik C, Augustynowicz E, Korzeniowska-Kowal A, Lutyhska A (2016) Application of the MALDI–TOF for identification of clostridium perfringens strains. Med Dosw Mikrobiol 68(1):13–21 - PubMed

MeSH terms

LinkOut - more resources