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. 2024 Feb 23;9(10):12254-12261.
doi: 10.1021/acsomega.4c00162. eCollection 2024 Mar 12.

Biological Activities of Galanthus fosteri Extracts: First Demonstration of the Interaction between Chlorogenic Acid and DNA Ligase by Molecular Docking

Safiye Elif Korcan  1 , Nevin Çankaya  1 , İbrahim Bulduk  2 , Gencer Güvercin  3 , Şah İsmail Çivi  4
Affiliations

Biological Activities of Galanthus fosteri Extracts: First Demonstration of the Interaction between Chlorogenic Acid and DNA Ligase by Molecular Docking

Safiye Elif Korcan et al. ACS Omega. .

Abstract

Within the Amaryllidaceae family, the bulbous plant species Galanthus fosteri (G. fosteri) belongs to the Galanthus genus. Alkaloids with a broad variety of biological functions are typically found in the flora of this family. The G. fosteri plant's organs' antioxidant activity, antibacterial impact, and antimicrobial qualities were examined in this study. Total flavonoid contents (TFC) and total phenolic contents (TPC) of plant extracts were measured with spectrophotometric methods, and antioxidant activity was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging technique. The HPLC method was used to determine the phenolic compounds on a component basis. The antibacterial properties of the extracts were assessed using the Kirby-Bauer disc diffusion method, and the minimum inhibitory concentration method against the pathogens Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Additionally, combination tests were performed between the extract and antibiotics. Leaf and stem extracts demonstrated greater antioxidant qualities than bulb extracts, despite the fact that extracts of plant organs did not exhibit appreciable levels of TPC, TFC, or antioxidant qualities. According to the HPLC analysis results, it was determined that chlorogenic acid was present in all of the extracts. In fact, it was determined that only chlorogenic acid was 8.02 (mg/10 g) in G. fosteri bulb peel, which has antimicrobial and antioxidant properties. A molecular docking study has demonstrated for the first time that the antibacterial effect of chlorogenic acid might be due to DNA replication inhibition.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Gallic acid calibration curve.
Figure 2
Figure 2
Catechin calibration curve.
Figure 3
Figure 3
HPLC chromatogram of phenolic standards.
Figure 4
Figure 4
Antimicrobial effect of G. fosteri by the disc diffusion technique.
Figure 5
Figure 5
Molecular coupling between chlorogenic acid and 1A0I (DNA ligase). The image on the left shows the binding position. The image on the right shows the molecular modeling of the interaction between chlorogenic acid’s 1A0I (DNA ligase) and amino acid residues.
Figure 6
Figure 6
Bond lengths in molecular docking between chlorogenic acid and 1A0I (DNA ligase).
See this image and copyright information in PMC

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