Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 May 1;179(2):407-12.
doi: 10.1042/bj1790407.

Adenosine triphosphate consumption by bacterial arginyl-transfer ribonucleic acid synthetases

J M Godeau, J Charlier

Adenosine triphosphate consumption by bacterial arginyl-transfer ribonucleic acid synthetases

J M Godeau et al. Biochem J. .

Abstract

ATP consumption by arginyl-tRNA synthetases from Escherichia coli and Bacillus stearothermophilus has been investigated by the firefly luciferin--luciferase assay. Arginyl-tRNA synthetase from E. coli utilizes ATP only for aminocylation of tRNA with a 1:1 stoicheiometry. In contrast, we have shown an adenosine triphosphatase activity of arginyl-tRNA synthetase from B. stearothermophilus in the absence of tRNAArg. Dowex chromatography revealed the formation of ADP by the thermophile enzyme; under aminoacylation conditions, AMP was also formed in amounts stoicheiometric with arginyl-tRNA formation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Proc Natl Acad Sci U S A. 1964 Aug;52:330-7 - PubMed
    1. Biochim Biophys Acta. 1963 Sep 17;76:48-53 - PubMed
    1. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1627-35 - PubMed
    1. J Biol Chem. 1961 May;236:1372-9 - PubMed
    1. Eur J Biochem. 1976 Nov 1;70(1):137-45 - PubMed

MeSH terms

Substances

LinkOut - more resources