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. 2024:2781:155-162.
doi: 10.1007/978-1-0716-3746-3_14.

Immunophenotyping of Leukocytes in Amniotic Fluid

Yi Xu  1 , Derek Miller  2 , Jose Galaz  3 , Nardhy Gomez-Lopez  4   5
Affiliations

Immunophenotyping of Leukocytes in Amniotic Fluid

Yi Xu et al. Methods Mol Biol. 2024.

Abstract

Immunophenotyping allows for the deep characterization of leukocytes present in biological samples. Here, we describe a complete procedure for the immunophenotyping of amniotic fluid, which can provide information into the immune processes taking place in the amniotic cavity. The protocol describes amniotic fluid cell count determination, processing, and the use of viability, extracellular antibody, and intracellular/intranuclear antibody staining prior to flow cytometer acquisition.

Keywords: Amniotic cavity; Flow cytometry; Immune cells; Pregnancy.

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