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. 2024 Mar 10;10(6):e27589.
doi: 10.1016/j.heliyon.2024.e27589. eCollection 2024 Mar 30.

Chemical profiling and arginine kinase inhibitory activity of Angelica dahurica leaves

Aitong Yang  1   2   3 , Junyan Zhang  4 , Guangying Lv  1 , Jiabao Chen  1 , Long Guo  1   2   3 , Yan Liu  5 , Yuguang Zheng  2   3   6 , Lei Wang  1   2   3
Affiliations

Chemical profiling and arginine kinase inhibitory activity of Angelica dahurica leaves

Aitong Yang et al. Heliyon. .

Abstract

Angelica dahurica is a medicinal herb of the Umbelliferae family. The dried root of A. dahurica, also known as Angelicae dahuricae Radix, is widely used in clinical treatment. However, the aboveground part of A. dahurica which accounted for over 70% of the total plant was abandoned in the field. In order to develop the value of the aboveground part of A. dahurica, the chemical constituents and arginine kinase (AK) inhibitory activity of A. dahurica leaves were studied. 85 volatile components were identified from A. dahurica leaves by GC-MS; 39 non-volatile components including sugars, amino acids and organic acids were identified by pre-column derivatization GC-MS analysis; and 7 coumarins were qualitatively and quantitatively analyzed by HPLC. Then, an inhibitory enzyme-linked immunosorbent assay (iEIA) was applied for evaluation of AK inhibitory activity. The extracts of A. dahurica leaves exhibited well inhibitory effects on AK. Further, potential AK inhibitors were screened by grey relational analysis and their inhibitory activities were validated by iEIA. l-aspartic acid exhibited strongest inhibitory effect on AK with its IC50 value was 0.558 mM, which was much lower than that of chlorpheniramine (6.644 mM). The obtained chemical profiles displayed chemical diversity of A. dahurica leaves and will provide data support for the future development and utilization of A. dahurica leaves. The screened potential AK inhibitors from A. dahurica leaves could be candidates for development of antiallergic substances or insecticides.

Keywords: Angelica dahurica leaves; Arginine kinase; Chemical composition; Correlation analysis.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
TIC of volatile metabolites in A. dahurica leaves by GC-MS; The number of peaks was consistent with those of compounds in Table 1.
Fig. 2
Fig. 2
TIC of nonvolatile metabolites in A. dahurica leaves by GC-MS after derivatization. The number of peaks was consistent with those of compounds in Table 2.
Fig. 3
Fig. 3
HPLC chromatograms of A. dahurica leaves (A) and reference substances mixture (B).
Fig. 4
Fig. 4
AK inhibition rates of A. dahurica leaves and chlorpheniramine (n = 3).
Fig. 5
Fig. 5
AK inhibition rates of 10 compounds comparing with chlorpheniramine at 2 mM (n = 3).
Fig. 6
Fig. 6
Inhibition rates of aspartic acid, lactic acid, citric acid, malic acid, shikimic acid and chlorpheniramine on AK.
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References

    1. Commission National Pharmacopoeia . Medical Science and Technology Press; China: 2020. Pharmacopoeia of the People's Republic of China.
    1. Tao HJ. Ben Cao Jing Ji Zhu.
    1. Lu F.C. Effect of Angelica dahurica leaves on bee sting. Shandong pharmaceut. ind. 1997;(3):56–57.
    1. Laino A., Lopez-Zavala A.A., Garcia-Orozco K.D., et al. Biochemical and structural characterization of a novel arginine kinase from the spider Polybetes pythagoricus. PeerJ. 2017;5:e3787. doi: 10.7717/peerj.3787. - DOI - PMC - PubMed
    1. Qian K., Guan Q.Q., Zhang H.Y., et al. RNAi-mediated knockdown of arginine kinase genes leads to high mortality and negatively affect reproduction and blood-feeding behavior of Culex pipiens pallens. PLoS Neglected Trop. Dis. 2022;16(11) doi: 10.1371/journal.pntd.0010954. - DOI - PMC - PubMed

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