Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Apr 2;121(14):e2402551121.
doi: 10.1073/pnas.2402551121. Epub 2024 Mar 25.

Phase separation promotes the activity of HECT E3 ligases-A word of caution

Daniela Eichbichler  1 , Christine Bernecker  1 , Florian Stengel  1 , Martin Scheffner  1
Affiliations

Phase separation promotes the activity of HECT E3 ligases-A word of caution

Daniela Eichbichler et al. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

Competing interests statement:The authors declare no competing interest.

Figures

Fig. 1.
Fig. 1.
1,6-HD inhibits E1 activity. Recombinant E1 (UBA1) was incubated with ubiquitin in the absence or presence of increasing concentrations of 1,6-HD or 2,5-HD as indicated at 37 °C for 30 min in the presence of pyrophosphatase (New England Biolabs, M2403L). E1 activity (i.e., ATP hydrolysis) was determined by the Malachite Green assay (Sigma-Aldrich, MAK307-1KT), normalized to the reaction in the absence of the respective HD (set to 100%), and the mean and SD were plotted using GraphPad Prism8 (n = 6).
See this image and copyright information in PMC

References

    1. Alberti S., Hyman A. A., Biomolecular condensates at the nexus of cellular stress, protein aggregation disease and ageing. Nat. Rev. Mol. Cell Biol. 22, 96–213 (2021). - PubMed
    1. Lyon A. S., Peeples W. B., Rosen M. K., A framework for understanding the functions of biomolecular condensates across scales. Nat. Rev. Mol. Cell Biol. 22, 215–235 (2021). - PMC - PubMed
    1. Luo Y. Y., Wu J. J., Li Y. M., Regulation of liquid-liquid phase separation with focus on post-translational modifications. Chem. Commun. (Camb) 57, 13275–13287 (2021). - PubMed
    1. Hou X. N., Tang C., The pros and cons of ubiquitination on the formation of protein condensates. Acta Biochim. Biophys. Sin. (Shanghai) 55, 1084–1098 (2023). - PMC - PubMed
    1. Komander D., Rape M., The ubiquitin code. Annu. Rev. Biochem. 81, 203–229 (2012). - PubMed

MeSH terms

Substances

LinkOut - more resources