Bosea beijingensis sp. nov., Telluria beijingensis sp. nov. and Agrococcus beijingensis sp. nov., isolated from baijiu mash

Abstract

The baijiu fermentation environment hosts a variety of micro-organisms, some of which still remain uncultured and uncharacterized. In this study, the isolation, cultivation and characterization of three novel aerobic bacterial strains are described. The cells of strain REN20^T were Gram-negative, strictly aerobic, motile and grew at 26-37 °C, at pH 6.0-9.0 and in the presence of 0-5.0 % (w/v) NaCl. The cells of strain REN29^T were Gram-negative, strictly aerobic, motile and grew at 15-30 °C, at pH 6.0-9.0 and in the presence of 0-10.0 % (w/v) NaCl. The cells of strain REN33^T were Gram-positive, strictly aerobic, motile and grew at 15-37 °C, at pH 5.0-10.0 and in the presence of 0-7.0 % (w/v) NaCl. The digital DNA-DNA hybridization and average nucleotide identity by orthology values between type strains in related genera and REN20^T (20.3-36.8 % and 79.8-89.9 %), REN29^T (20.3-36.8 % and 74.5-88.5 %) and REN33^T (22.6-48.6 % and 75.8-84.2 %) were below the standard cut-off criteria for the delineation of bacterial species, respectively. Based on polyphasic taxonomy analysis, we propose three new species, Bosea beijingensis sp. nov. (=REN20^T=GDMCC 1.2894^T=JCM 35118^T), Telluria beijingensis sp. nov. (=REN29^T=GDMCC 1.2896^T=JCM 35119^T) and Agrococcus beijingensis sp. nov. (=REN33^T=GDMCC 1.2898^T=JCM 35164^T), which were recovered during cultivation and isolation from baijiu mash.

Keywords: Agrococcus beijingensis; Bosea beijingensis; Telluria beijingensis; baijiu mash; polyphasic taxonomy.