Shigella Detection and Molecular Serotyping With a Customized TaqMan Array Card in the Enterics for Global Health (EFGH): Shigella Surveillance Study

doi:10.1093/ofid/ofad574

. 2024 Mar 25;11(Suppl 1):S34-S40.

doi: 10.1093/ofid/ofad574. eCollection 2024 Mar.

Shigella Detection and Molecular Serotyping With a Customized TaqMan Array Card in the Enterics for Global Health (EFGH): Shigella Surveillance Study

Jie Liu¹, Paul F Garcia Bardales², Kamrul Islam³, Sheikh Jarju⁴, Jane Juma⁵, Chimwemwe Mhango⁶, Queen Naumanga⁷, Sonia Qureshi⁸, Catherine Sonye⁹, Naveed Ahmed⁸, Fatima Aziz⁸, Md Taufiqur Rahman Bhuiyan³, Mary Charles⁶, Nigel A Cunliffe¹⁰, Mahamadou Abdou⁵, Sean R Galagan¹¹, Ensa Gitteh⁴, Ibrehima Guindo⁵, M Jahangir Hossain⁴, Abdoulie M J Jabang⁴, Khuzwayo C Jere^{6

10

12}, Flywell Kawonga⁶, Mariama Keita⁴, Noumou Yakhouba Keita⁵, Karen L Kotloff^{13

14

15}, Wagner V Shapiama Lopez², Stephen Munga⁹, Maribel Paredes Olortegui², Richard Omore⁹, Patricia B Pavlinac¹¹, Firdausi Qadri³, Farah Naz Qamar⁸, S M Azadul Alam Raz³, Laura Riziki⁹, Francesca Schiaffino^{7

16}, Suzanne Stroup⁷, Sarata Nassoun Traore⁵, Tackeshy Pinedo Vasquez², Mohammad Tahir Yousafzai⁸, Martin Antonio^{4

17

18}, Jennifer E Cornick^{6

10}, Furqan Kabir⁸, Farhana Khanam³, Margaret N Kosek⁷, John Benjamin Ochieng⁹, James A Platts-Mills⁷, Sharon M Tennant^{13

14}, Eric R Houpt⁷

Affiliations

¹ School of Public Health, Qingdao University, Qingdao, China.
² Asociación Benéfica PRISMA, Iquitos, Loreto, Peru.
³ Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
⁴ Medical Research Council Unit The Gambia, London School of Hygiene and Tropical Medicine, Fajara, The Gambia.
⁵ Centre pour le Développement des Vaccins du Mali (CVD-Mali), Bamako, Mali.
⁶ Malawi Liverpool Wellcome Research Programme, Blantyre, Malawi.
⁷ Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA.
⁸ Department of Pediatrics and Child Health, The Aga Khan University, Karachi, Pakistan.
⁹ Center for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya.
¹⁰ Institute of Infection, Veterinary and Ecological Sciences, Department of Clinical Infection, Microbiology and Immunology, University of Liverpool, Liverpool, United Kingdom.
¹¹ Department of Global Health, University of Washington, Seattle, Washington, USA.
¹² Department of Medical Laboratory Sciences, School of Life Sciences and Health Professions, Kamuzu University of Health Sciences, Blantyre, Malawi.
¹³ Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
¹⁴ Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
¹⁵ Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA.
¹⁶ Faculty of Veterinary Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru.
¹⁷ Centre for Epidemic Preparedness and Response, London School of Hygiene & Tropical Medicine, London, UK.
¹⁸ Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.

PMID: 38532960
PMCID: PMC10962731
DOI: 10.1093/ofid/ofad574

Shigella Detection and Molecular Serotyping With a Customized TaqMan Array Card in the Enterics for Global Health (EFGH): Shigella Surveillance Study

Jie Liu et al. Open Forum Infect Dis. 2024.

. 2024 Mar 25;11(Suppl 1):S34-S40.

doi: 10.1093/ofid/ofad574. eCollection 2024 Mar.

Authors

Affiliations

¹ School of Public Health, Qingdao University, Qingdao, China.
² Asociación Benéfica PRISMA, Iquitos, Loreto, Peru.
³ Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
⁴ Medical Research Council Unit The Gambia, London School of Hygiene and Tropical Medicine, Fajara, The Gambia.
⁵ Centre pour le Développement des Vaccins du Mali (CVD-Mali), Bamako, Mali.
⁶ Malawi Liverpool Wellcome Research Programme, Blantyre, Malawi.
⁷ Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA.
⁸ Department of Pediatrics and Child Health, The Aga Khan University, Karachi, Pakistan.
⁹ Center for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya.
¹⁰ Institute of Infection, Veterinary and Ecological Sciences, Department of Clinical Infection, Microbiology and Immunology, University of Liverpool, Liverpool, United Kingdom.
¹¹ Department of Global Health, University of Washington, Seattle, Washington, USA.
¹² Department of Medical Laboratory Sciences, School of Life Sciences and Health Professions, Kamuzu University of Health Sciences, Blantyre, Malawi.
¹³ Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
¹⁴ Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
¹⁵ Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA.
¹⁶ Faculty of Veterinary Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru.
¹⁷ Centre for Epidemic Preparedness and Response, London School of Hygiene & Tropical Medicine, London, UK.
¹⁸ Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.

PMID: 38532960
PMCID: PMC10962731
DOI: 10.1093/ofid/ofad574

Abstract

Background: Quantitative polymerase chain reaction (qPCR) targeting ipaH has been proven to be highly efficient in detecting Shigella in clinical samples compared to culture-based methods, which underestimate Shigella burden by 2- to 3-fold. qPCR assays have also been developed for Shigella speciation and serotyping, which is critical for both vaccine development and evaluation.

Methods: The Enterics for Global Health (EFGH) Shigella surveillance study will utilize a customized real-time PCR-based TaqMan Array Card (TAC) interrogating 82 targets, for the detection and differentiation of Shigella spp, Shigella sonnei, Shigella flexneri serotypes, other diarrhea-associated enteropathogens, and antimicrobial resistance (AMR) genes. Total nucleic acid will be extracted from rectal swabs or stool samples, and assayed on TAC. Quantitative analysis will be performed to determine the likely attribution of Shigella and other particular etiologies of diarrhea using the quantification cycle cutoffs derived from previous studies. The qPCR results will be compared to conventional culture, serotyping, and phenotypic susceptibility approaches in EFGH.

Conclusions: TAC enables simultaneous detection of diarrheal etiologies, the principal pathogen subtypes, and AMR genes. The high sensitivity of the assay enables more accurate estimation of Shigella-attributed disease burden, which is critical to informing policy and in the design of future clinical trials.

Keywords: PCR; ipaH; quantification; rectal swab; serotyping.

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Conflict of interest statement

Potential conflicts of interest. All authors: No reported conflicts.

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References

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[1] Hale TL, Keusch GT, Baron S, Baron S. Shigella. In: Baron S, eds. Medical microbiology, 4th ed. Galveston: University of Texas Medical Branch at Galveston, 1996:Chapter 22, 1.

[2] Hale TL, Keusch GT, Baron S, Baron S. Shigella. In: Baron S, eds. Medical microbiology, 4th ed. Galveston: University of Texas Medical Branch at Galveston, 1996:Chapter 22, 1.

[3] Kotloff KL, Winickoff JP, Ivanoff B, et al. Global burden of Shigella infections: implications for vaccine development and implementation of control strategies. Bull World Health Organ 1999; 77:651–66. - PMC - PubMed

[4] Kotloff KL, Winickoff JP, Ivanoff B, et al. Global burden of Shigella infections: implications for vaccine development and implementation of control strategies. Bull World Health Organ 1999; 77:651–66. - PMC - PubMed

[5] Livio S, Strockbine NA, Panchalingam S, et al. Shigella isolates from the Global Enteric Multicenter Study inform vaccine development. Clin Infect Dis 2014; 59:933–41. - PMC - PubMed

[6] Livio S, Strockbine NA, Panchalingam S, et al. Shigella isolates from the Global Enteric Multicenter Study inform vaccine development. Clin Infect Dis 2014; 59:933–41. - PMC - PubMed

[7] Muzembo BA, Kitahara K, Mitra D, et al. Burden of Shigella in South Asia: a systematic review and meta-analysis. J Travel Med 2023; 30:taac132. - PubMed

[8] Muzembo BA, Kitahara K, Mitra D, et al. Burden of Shigella in South Asia: a systematic review and meta-analysis. J Travel Med 2023; 30:taac132. - PubMed

[9] Sun Q, Lan R, Wang Y, et al. Development of a multiplex PCR assay targeting O-antigen modification genes for molecular serotyping of Shigella flexneri. J Clin Microbiol 2011; 49:3766–70. - PMC - PubMed

[10] Sun Q, Lan R, Wang Y, et al. Development of a multiplex PCR assay targeting O-antigen modification genes for molecular serotyping of Shigella flexneri. J Clin Microbiol 2011; 49:3766–70. - PMC - PubMed

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Shigella Detection and Molecular Serotyping With a Customized TaqMan Array Card in the Enterics for Global Health (EFGH): Shigella Surveillance Study

Affiliations

Shigella Detection and Molecular Serotyping With a Customized TaqMan Array Card in the Enterics for Global Health (EFGH): Shigella Surveillance Study

Authors

Affiliations

Abstract

Conflict of interest statement

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Cited by

References

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LinkOut - more resources

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