Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Aug;87(8):1836-1848.
doi: 10.1002/jemt.24563. Epub 2024 Mar 27.

Altered glycosylation in secreting cells of the gastric glands of aquaporin-4-deficient mice

Donatella Mentino  1 , Grazia Paola Nicchia  1 , Antonio Frigeri  2 , Salvatore Desantis  3 , Marco Vito Guglielmi  1 , Daniela Semeraro  1 , Giovanni Scillitani  1 , Maria Mastrodonato  1
Affiliations

Altered glycosylation in secreting cells of the gastric glands of aquaporin-4-deficient mice

Donatella Mentino et al. Microsc Res Tech. 2024 Aug.

Abstract

Aquaporins (AQPs) are important for water transport in the gastrointestinal tract. Changes in their expression and/or localization could cause in disorders and be used as therapeutic targets. Aquaporin-4 (AQP4) is expressed predominantly on the basolateral membrane of the parietal cells in the corpus of the murine gastric glands. Although the secretion of gastric juice is not affected in AQP4-deficient knockout, we evaluated by light microscopy whether the lack of AQP4 affects the glycopatterns of secreting gastric cells. Wild type (WT) and AQP4-deficient knockout mice (KO) were fed a standard diet ad libitum before sacrifice. Segments of stomach corpus were collected, fixed in buffered formalin, and embedded in paraffin wax. Sections, 5-μm thick, were analyzed by histochemical methods (Periodic acid-Schiff, Alcian Blue pH 2.5), and binding of lectins specific to GalNAc (SBA, DBA), Gal (PNA) GlcNAc (WGA, GSAII) mannose and/or glucose (ConA), and fucose (UEA-I, AAA, LTA). Immunohistochemical methods such as anti-Muc6 for neck cells and anti- β- H+/K+-ATPase for parietal cells were also performed. Compared to WT mice, in the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated, and fucosylated residues were observed; lower fucosylation resulted also in the parietal cells. The observed differences of KO in respect to WT could lead to severer pathological conditions. RESEARCH HIGHLIGHTS: Glycopatterns in gastric glands were compared between wild type (WT) and AQP4-deficient knockout (KO) mice by histochemical and lectin-binding methods. In the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated and fucosylated residues were observed. In the parietal cells lower fucosylation also resulted. AQP4-deficiency affects glycosylation and could result in altered functionality and pathological conditions.

Keywords: AQP4‐deficient mouse; gastric glands; glycopattern analysis; lectin histochemistry.

PubMed Disclaimer

References

REFERENCES

    1. Bodis, B., Nagy, G., Nemeth, P., & Mozsik, G. (2001). Active water selective channels in the stomach: Investigation of aquaporins after ethanol and capsaicin treatment in rats. Journal of Physiology, Paris, 95, 271–275.
    1. Carlucci, R., Cipriano, G., Fanizza, C., Gerussi, T., Maglietta, R., Petrella, A., Pietroluongo, G., Ricci, P., Semeraro, D., Guglielmi, M. V., Scillitani, G., & Mentino, D. (2022). Glycopatterns of the foregut in the striped dolphin Stenella coeruleoalba, Meyen 1833 from the Mediterranean Sea. Marine Mammal Science, 38, 190–211.
    1. Carlucci, R., Mentino, D., Semeraro, D., Ricci, P., Sion, L., & Scillitani, G. (2019). Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes). Journal of Fish Biology, 94(1), 122–131.
    1. Carmosino, M., Mazzone, A., Laforenza, U., Gastaldi, G., Svelto, M., & Valenti, G. (2005). Altered expression of aquaporin 4 and H(+)/K(+)‐ATPase in the stomachs of peptide YY (PYY) transgenic mice. Biology of the Cell, 97, 735–742.
    1. Chow, D. C., Browning, C. M., & Forte, J. G. (1992). Gastric H(+)‐K(+)‐ATPase activity is inhibited by reduction of disulfide bonds in beta‐subunit. The American Journal of Physiology, 263, C39–C46.

LinkOut - more resources