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. 2024 May 9;13(5):e0010224.
doi: 10.1128/mra.00102-24. Epub 2024 Mar 27.

Microbial genome collection of aerobic granular sludge cultivated in sequencing batch reactors using different carbon source mixtures

Jaspreet S Saini #  1 , Aline Adler #  1 , Laëtitia Cardona  1 , Pilar Natalia Rodilla Ramírez  1 , Ruizhe Pei  1 , Christof Holliger  1
Affiliations

Microbial genome collection of aerobic granular sludge cultivated in sequencing batch reactors using different carbon source mixtures

Jaspreet S Saini et al. Microbiol Resour Announc. .

Abstract

Aerobic granular sludge (AGS) consists of a microbial consortium that has an important role in wastewater treatment. This study investigates AGS microorganisms cultivated in a laboratory-scale sequencing batch reactor. Metagenomic sequencing was conducted using PacBio and Illumina, resulting in 759 metagenome-assembled genomes, 331 of which remained after dereplication.

Keywords: PacBio; accumulibacter; aerobic granular sludge; biofilms; illumina; metagenome-assembled genomes; wastewater.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
A summary of the MAGs collection before (n = 759) and after dereplication (n = 331) as described in two panels. It includes BBMap-based statistics and CheckM-based quality assessment. The statistics comprise (A-B) genome size and (C-F) N50 and L50 values. (G-J) Assessment of MAGs completion and contamination by CheckM. The colors indicate the different strategies described as workflows in methods.
See this image and copyright information in PMC

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