Agroindustrial By-Products as a Source of Biostimulants Enhancing Responses to Abiotic Stress of Horticultural Crops

Abstract

Together with other abiotic stresses such as drought and high temperatures, salt stress is one of the most deleterious environmental factors affecting plant development and productivity, causing significant crop yield reductions. The progressive secondary salinisation of irrigated farmland is a problem as old as agriculture but is aggravated and accelerated in the current climate change scenario. Plant biostimulants, developed commercially during the last decade, are now recognised as innovative, sustainable agronomic tools for improving crop growth, yield, plant health and tolerance to abiotic stress factors such as water and soil salinity. Biostimulants are a disparate collection of biological extracts, natural and synthetic organic compounds or mixtures of compounds, inorganic molecules and microorganisms, defined by the positive effects of their application to crops. The growing interest in biostimulants is reflected in the increasing number of scientific reports published on this topic in recent years. However, the processes triggered by the biostimulants and, therefore, their mechanisms of action remain elusive and represent an exciting research field. In this review, we will mainly focus on one specific group of biostimulants, protein hydrolysates, generally produced from agricultural wastes and agroindustrial by-products-contributing, therefore, to more sustainable use of resources and circular economy-and primarily on the consequences of their application on the abiotic stress resistance of horticultural crops. We will summarise data in the scientific literature describing the biostimulants' effects on basic, conserved mechanisms activated in response to elevated salinity and other abiotic stress conditions, such as the control of ion transport and ion homeostasis, the accumulation of osmolytes for osmotic adjustment, or the activation of enzymatic and non-enzymatic antioxidant systems to counteract the induced secondary oxidative stress. The collected information confirms the positive effects of biostimulants on crop tolerance to abiotic stress by enhancing morphological, physiological and biochemical responses, but also highlights that more work is needed to further establish the molecular mechanisms underlying biostimulants' effects.

Keywords: antioxidant systems; circular economy; ionic stress; molecular mechanisms; osmotic stress; oxidative stress; salt tolerance; stress markers.

Figure 4

Protein hydrolysates derived from agroindustrial waste of plant or animal origin and their effects on improving crop abiotic stress tolerance. Adapted from Colla et al. [68] and Sun et al. [96].

Figure 1

Biostimulant categories, defined according to the sources from which they are produced (Based on European Commission [36]).

Figure 2

Venn diagram of the mechanisms induced by different biostimulants, classified according to their origin—microbial, non-microbial and waste-derived—contributing to enhancing plant tolerance to abiotic stress, highlighting the extensive overlapping of the effects of biostimulants belonging to the three groups. Prepared from information in references [42,58].

Figure 3

Lactuca sativa roots under salt stress conditions (50 mM NaCl). (a) Plants treated with BALOX^®, a biostimulant based on plant protein hydrolysates and (b) control, non-treated plants; see [89].