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. 2024 Mar 21;16(6):912.
doi: 10.3390/nu16060912.

Genistein Supplementation and Bone Health in Breast Cancer in Rats

Dorota Skrajnowska  1 , Wojciech Bielecki  2 , Arkadiusz Szterk  3   4 , Karol Ofiara  3   4 , Barbara Bobrowska-Korczak  1
Affiliations

Genistein Supplementation and Bone Health in Breast Cancer in Rats

Dorota Skrajnowska et al. Nutrients. .

Abstract

The aim of our study was to analyse the effect of supplementation with various forms of genistein (nano-, micro-, and macro-) on the mineral status of rat femurs in conditions of DMBA-induced mammary gland neoplasia. Thirty-two 30-day-old Sprague Dawley rats were used in the study. The rats were divided into four experimental groups: a control group (without supplementation) and groups supplemented with nanosized (92 ± 41 nm), microsized (587 ± 83 nm), and macrosized genistein. Micromorphometric and histological examination of the rat femurs were performed, as well as analysis of the weight and mineral composition (17 elements). Quadrupole ICP-MS was used for analysis of all trace elements. Supplementation with genistein (nano-, micro-, and macro-) was shown to cause changes in the mineral composition of the bones. In the rats receiving nanogenistein, disintegration of the bone tissue was observed. The femurs of these animals had higher content of calcium (by nearly 300%) and potassium (by 25%) than the other groups, while the level of magnesium was about 22% lower. In the case of microelements, there were increases in copper (by 67%), boron (48%), manganese (13%), and nickel (100%), and a 16% decrease in strontium compared to the bones of rats without genistein supplementation. Changes in micromorphometric parameters, resulting in increased bone fragility, were observed. Administration of genistein was found to have an effect on the amount of trace elements in the bone tissue of rats with breast cancer.

Keywords: bones; cancer; genistein; mineral composition.

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Conflict of interest statement

A.S. and K.O. were employed by ASLab Science. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Scheme of experiment design.
Figure 2
Figure 2
Results of the histopathological analysis of rat femurs in conditions of neoplasia in relation to supplementation (macro-, micro-, or nanogenistein) (1—compact layer; 2—plexiform bone; 3—medullary cavity) (haematoxylin & eosin staining, 20× magnification).
Figure 3
Figure 3
Calcium in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 4
Figure 4
Potassium in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 5
Figure 5
Magnesium in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 6
Figure 6
Strontium in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 7
Figure 7
Copper in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 8
Figure 8
Manganese in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 9
Figure 9
Sodium in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 10
Figure 10
Boron in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 11
Figure 11
Nickel in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 12
Figure 12
Arsenic in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 13
Figure 13
Barium in the femurs of rats supplemented with various forms of genistein: macrogenistein; microgenistein; nanogenistein; no supplementation (standard diet).
Figure 14
Figure 14
Heat map of correlations of 17 elements in the bones of rats receiving the standard diet (blue indicates positive correlations and red indicates negative correlations).
Figure 15
Figure 15
Heat map of correlations of 17 elements in the bones of rats receiving macrogenistein (blue indicates positive correlations and red indicates negative correlations).
Figure 16
Figure 16
Heat map of correlations of 17 elements in the bones of rats receiving microgenistein (blue indicates positive correlations and red indicates negative correlations).
Figure 17
Figure 17
Heat map of correlations of 17 elements in the bones of rats receiving nanogenistein (blue indicates positive correlations and red indicates negative correlations).
See this image and copyright information in PMC

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