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. 2024 Jun;109(2):116233.
doi: 10.1016/j.diagmicrobio.2024.116233. Epub 2024 Feb 28.

Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

Anja Lindis Dahl  1 , Martin Barfred Friis  2 , Hanne Wiese Hallberg  2 , Gitte Qvist Kristiansen  3 , Barbara Juliane Holzknecht  4
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Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

Anja Lindis Dahl et al. Diagn Microbiol Infect Dis. 2024 Jun.
Free article

Abstract

Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.

Keywords: Carriage; Enrichment; VRE; Vancomycin-resistant enterococci; vanB.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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