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. 2024:2758:319-329.
doi: 10.1007/978-1-0716-3646-6_17.

Analysis of the Snake Venom Peptidome

Solange M T Serrano  1 , André Zelanis  2 , Jackson G Miyamoto  3 , Jackelinne Y Hayashi  3 , Eduardo S Kitano  1 , Alexandre K Tashima  4
Affiliations

Analysis of the Snake Venom Peptidome

Solange M T Serrano et al. Methods Mol Biol. 2024.

Abstract

Snake venom peptidomes are known to be a large source of molecules with different pharmacological properties. The complexity and variability of snake venoms, the presence of proteinases, and the lack of complete species-specific genome sequences make snake venom peptidome profiling a challenging task that requires especial technical strategies for sample processing and mass spectrometric analysis. Here, we describe a method for assessing the content of snake venom peptides and highlight the importance of sampling procedures, as they substantially influence the peptidomic complexity of snake venoms.

Keywords: Mass spectrometry; Peptidome; Proteinase inhibition; Snake venom; Solid-phase extraction.

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