Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Mar 30;259(5):106.
doi: 10.1007/s00425-024-04377-3.

OsmiR5519 regulates grain size and weight and down-regulates sucrose synthase gene RSUS2 in rice (Oryza sativa L.)

Shengyuan Guo #  1 , Yajuan Li #  2 , Yan Wang  1 , Yangwen Xu  1 , Yuting Li  1 , Ping Wu  1 , Jinwen Wu  3   4   1 , Lan Wang  3   4   1 , Xiangdong Liu  5   6   7   8 , Zhixiong Chen  9   10   11   12
Affiliations

OsmiR5519 regulates grain size and weight and down-regulates sucrose synthase gene RSUS2 in rice (Oryza sativa L.)

Shengyuan Guo et al. Planta. .

Abstract

The up-regulation of OsmiR5519 results in the decrease of grain size, weight and seed setting rate. OsmiR5519 plays important roles in the process of grain filling and down-regulates sucrose synthase gene RSUS2. MicroRNAs (miRNAs) are one class of small non-coding RNAs that act as crucial regulators of plant growth and development. In rice, the conserved miRNAs were revealed to regulate the yield components, but the function of rice-specific miRNAs has been rarely studied. The rice-specific OsmiR5519 was found to be abundantly expressed during reproductive development, but its biological roles remain unknown. In this study, the function of rice-specific OsmiR5519 was characterized with the miR5519-overexpressing line (miR5519-OE) and miR5519-silenced line (STTM5519). At seedling stage, the content of sucrose, glucose and fructose was obviously lower in the leaves of miR5519-OE lines than those of wild-type (WT) line. The grain size and weight were decreased significantly in miR5519-OE lines, compared to those of WT rice. The cell width of hull in miR5519-OE was smaller than that in WT. The seed setting rate was notably reduced in miR5519-OE lines, but not in STTM5519 lines. Cytological observation demonstrated that the inadequate grain filling was the main reason for the decline of seed setting rate in miR5519-OE lines. The percentage of the defects of grain amounted to 40% in miR5519-OE lines, which almost equaled to the decreased value of seed setting rate. Furthermore, the sucrose synthase gene RSUS2 was identified as a target of OsmiR5519 via RNA ligase-mediated 3'-amplification of cDNA ends (3'-RLM-RACE), dual luciferase assays and transient expression assays. In summary, our results suggest that OsmiR5519 regulates grain size and weight and down-regulates RSUS2 in rice.

Keywords: Grain filling; Grain size; Grain weight; Rice-specific miRNA; Seed setting rate.

PubMed Disclaimer

References

    1. Abid G, Silue S, Muhovski Y et al (2009) Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development. Gene 439:1–10. https://doi.org/10.1016/j.gene.2009.03.007 - DOI - PubMed
    1. Asano T, Kunieda N, Omura Y et al (2002) Rice SPK, a calmodulin-like domain protein kinase, is required for storage product accumulation during seed development: phosphorylation of sucrose synthase is a possible factor. Plant Cell 14:619–628. https://doi.org/10.1105/tpc.010454 - DOI - PubMed - PMC
    1. Bai X, Wu B, Xing Y (2012) Yield-related QTLs and their applications in rice genetic improvement. J Integr Plant Biol 54:300–311. https://doi.org/10.1111/j.1744-7909.2012.01117.x - DOI - PubMed
    1. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297. https://doi.org/10.1016/S0092-8674(04)00045-5 - DOI - PubMed
    1. Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136:215–233. https://doi.org/10.1016/j.cell.2009.01.002 - DOI - PubMed - PMC

LinkOut - more resources