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. 2024 Oct;18(4):1062-1068.
doi: 10.1055/s-0044-1779425. Epub 2024 Mar 31.

Potency of Hyperbaric Oxygen Therapy on Oral Candidiasis Diabetes Mellitus

Kristanti Parisihni  1 , Dian Mulawarmanti  1 , Kharinna Widowati  2
Affiliations

Potency of Hyperbaric Oxygen Therapy on Oral Candidiasis Diabetes Mellitus

Kristanti Parisihni et al. Eur J Dent. 2024 Oct.

Abstract

Objectives: This study aimed to determine the potency of hyperbaric oxygen therapy (HBOT) to the blood glucose level, amount of lymphocytes, and the expression of antibody anti-Candida in oral candidiasis diabetes mellitus.

Materials and methods: Thirty Wistar rats were divided into five groups: K1 normal-control group, while K2, P1, P2, and P3 were diabetes induced by streptozotocin 50 mg/kg body weight. Oral candidiasis was induced by inoculation 0.1 mL Candida albicans ATCC 10321 on dorsum of the tongue. P1 was given nystatin oral suspension, P2 was treated by HBOT 2.4 ATA, 3 × 30 minutes each with a 5-minute interval, and P3 was treated by the combination of nystatin and HBOT. All the treatments were performed for 5 consecutive days. Fasting blood glucose level was examined on glucometer strips, lymphocyte was examined from complete blood count, and the expression of antibody anti-Candida was examined on immunohistochemistry staining STATISTICAL ANALYSIS: Data analyzed by a one-way analysis of variance and least significant difference test with the result of p-value less than 0.05 was considered to be statistically significant.

Results: HBOT reduced blood glucose level (p > 0.05) increased the amount of lymphocyte (p < 0.05). All treatments reduced the expression of antibody anti-Candida (p < 0.05) with the best result on combination of HBOT with nystatin.

Conclusion: HBOT decreased the blood glucose level, increased the amount of lymphocytes, and reduced the expression of antibody anti-Candida in oral candidiasis diabetes mellitus.

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Conflict of interest statement

None declared.

Figures

Fig. 1
Fig. 1
Mean of blood glucose level (mg/dL), before streptozotocin induction, after streptozotocin induction, and at the termination time in oral candidiasis diabetes mellitus rats.
Fig. 2
Fig. 2
Mean of lymphocyte count in all groups.
Fig. 3
Fig. 3
Immunohistochemistry staining on tongue slide of the rats. Healthy normal subject showed no expression of antibody anti- Candida (K1), The black arrows indicate the expression of antibody anti-Candida on untreated oral candidiasis diabetes mellitus (K2) and in all treatment groups (P1, P2, P3) (at 400x magnification).
Fig. 4
Fig. 4
Mean expression of antibody anti- Candida in all groups of oral candidiasis diabetes mellitus.
See this image and copyright information in PMC

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