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. 2024 Mar 22;21(1):e20230146.
doi: 10.1590/1984-3143-AR2023-0146. eCollection 2024.

Padi6 expression patterns in buffalo oocytes and preimplantation embryos

Qinqiang Sun  1 , Yuan Yang  1 , Yue Zhang  1 , Dongrong Chen  1 , Haiying Zheng  2 , Guangsheng Qin  2 , Qiang Fu  1
Affiliations

Padi6 expression patterns in buffalo oocytes and preimplantation embryos

Qinqiang Sun et al. Anim Reprod. .

Abstract

The subcortical maternal complex, which consists of maternal-effect genes, plays a crucial role in the development of oocytes and preimplantation embryo until the activation of the zygote genome. One such gene, known as peptidyl-arginine deiminase VI (Padi6), is involved in the oocyte maturation, fertilization and embryonic development. However, the precise function of Padi6 gene in buffalo is still unclear and requires further investigation. In this study, the sequence, mRNA and protein expression patterns of Padi6 gene were analyzed in oocytes, preimplantation embryos and somatic tissues of buffalo. The coding sequence of gene was successfully cloned and characterized. Real-time quantitative PCR results indicated an absence of Padi6 transcripts in somatic tissues. Notably, the expression levels of Padi6 in oocytes showed an increased from the germinal vesicle stage to metaphase II stage, followed by a rapid decrease during the morula and blastocyst stages. Immunofluorescence analysis confirmed these findings, revealing a noticeable decline in protein expression levels. Our research provides the initial comprehensive expression profile of Padi6 in buffalo oocytes and preimplantation embryos, serving as a solid foundation for further investigations into the functionality of maternal-effect genes in buffalo.

Keywords: Padi6; immunofluorescence; maternal-effect genes; oocyte; preimplantation embryo.

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Conflict of interest statement

Conflicts of interest: The authors have no conflict of interest to declare.

Figures

Figure 1
Figure 1. Electrophoresis results of PCR amplification products. M: DNA Marker; 1: blank control; 2: 576-bp fragment; 3: 712-bp fragment; 4: 639-bp fragment; 5: 508-bp fragment.
Figure 2
Figure 2. Sequence homology analysis of different mammalian species.
Figure 3
Figure 3. Padi6 mRNA expression profile based on qRT-PCR of multiple tissues. 1: heart, 2: liver, 3: spleen, 4: lung, 5: kidney, 6: brain, 7: spinal cord, 8: tongue, 9: throat, 10: stomach, 11: muscle, 12: fat, 13: testicle, 14: uterus, 15: ovary, 16: oocyte.
Figure 4
Figure 4. Padi6 mRNA expression levels in buffalo oocytes and preimplantation embryos. **Compared with control group, P<0.01.
Figure 5
Figure 5. Immunofluorescence of PADI6 protein in buffalo oocytes and preimplantation embryos.
See this image and copyright information in PMC

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