Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1985 Mar;82(6):1653-7.
doi: 10.1073/pnas.82.6.1653.

Cloning and nucleotide sequence determination of the Clostridium pasteurianum ferredoxin gene

M C Graves, G T Mullenbach, J C Rabinowitz
Comparative Study

Cloning and nucleotide sequence determination of the Clostridium pasteurianum ferredoxin gene

M C Graves et al. Proc Natl Acad Sci U S A. 1985 Mar.

Abstract

We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain greater than 6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobase-pair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1981 Jun 11;9(11):2577-88 - PubMed
    1. Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 - PubMed
    1. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed
    1. Nature. 1972 Jan 7;235(5332):40-2 - PubMed
    1. J Bacteriol. 1972 Dec;112(3):1057-69 - PubMed

Publication types

Associated data

LinkOut - more resources