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. 1985 Apr;82(7):1954-8.
doi: 10.1073/pnas.82.7.1954.

Characterization of a yeast mitochondrial promoter by deletion mutagenesis

T K Biswas, J C Edwards, M Rabinowitz, G S Getz

Characterization of a yeast mitochondrial promoter by deletion mutagenesis

T K Biswas et al. Proc Natl Acad Sci U S A. 1985 Apr.

Abstract

We have generated collections of mutants of the promoter for the small rRNA gene from the mitochondria of yeast deleted from either the 3' or 5' end. Plasmids containing the partially deleted promoter were assayed for their ability to direct correct transcriptional initiation in a homologous in vitro system. We find that the region required for high-efficiency promoter function lies between positions -10 and +2. Our methods detected no effect of flanking sequences on the strength of this promoter.

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References

    1. Cell. 1980 Jan;19(1):27-35 - PubMed
    1. Annu Rev Genet. 1979;13:319-53 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. J Biol Chem. 1981 Feb 10;256(3):1466-72 - PubMed
    1. J Biol Chem. 1981 Feb 10;256(3):1474-81 - PubMed

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