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. 2024 May 6;86(5):468-473.
doi: 10.1292/jvms.23-0526. Epub 2024 Apr 2.

Antimicrobial resistance patterns of Streptococcus uberis isolates from bovine milk in Chiba prefecture, Japan: association between multidrug resistance and clonal complex 996

Yuzo Tsuyuki  1   2   3 , Takahiro Maeda  2 , Kae Torii  1 , Haruno Yoshida  2 , Noriaki Ikeda  4 , Saki Yoshida  4 , Masahiko Ito  5 , Mieko Goto  2 , Takashi Takahashi  2
Affiliations

Antimicrobial resistance patterns of Streptococcus uberis isolates from bovine milk in Chiba prefecture, Japan: association between multidrug resistance and clonal complex 996

Yuzo Tsuyuki et al. J Vet Med Sci. .

Abstract

Streptococcus uberis is one of major pathogens causing bovine mastitis. However, there is poor information on antimicrobial resistance (AMR) among the Japanese isolates. To provide treatment information for the mastitis caused by S. uberis in Japan, we aimed to clarify AMR patterns of the isolates from bovine milk mainly in Chiba. AMR phenotyping/genotyping [blaZ-erm(A)-erm(B)-mef(A)-linB-lnuD-tet(M)-tet(O)-tet(K)-tet(L)-tet(S)] and multilocus sequence typing were performed to analyze relationships between AMR patterns and clonal complexes (CCs). Resistance to tetracycline-, macrolide-, and lincosamide-classes was mainly associated with possession of tet(O), tet(S), erm(B), linB, and lnuD genes. CC996 was significantly associated with multidrug resistance (P<0.0001). These findings will aid Chiba farm animal clinics in treating bovine mastitis.

Keywords: Chiba; Streptococcus uberis; antimicrobial resistance; bovine milk; clonal complex.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

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