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. 2024 Mar 20:15:1382781.
doi: 10.3389/fmicb.2024.1382781. eCollection 2024.

Study on the effect and mechanism of Lacticaseibacillus rhamnosus AFY06 on inflammation-associated colorectal cancer induced by AOM/DSS in mice

Jing Zhang #  1 , Piyun Zhang #  2 , Sijia Li  1 , Ting Yu  3 , Xiangyu Lai  4 , Yongpeng He  4
Affiliations

Study on the effect and mechanism of Lacticaseibacillus rhamnosus AFY06 on inflammation-associated colorectal cancer induced by AOM/DSS in mice

Jing Zhang et al. Front Microbiol. .

Abstract

Introduction: Lacticaseibacillus rhamnosus AFY06 (LR-AFY06) is a microorganism isolated from naturally fermented yogurt in Xinjiang, China.

Methods: In this study, we investigated the effects and mechanisms of LR-AFY06 in a mouse model of inflammation-associated colon cancer. The mouse model was established by azoxymethane/dextran sulfate sodium (AOM/DSS) induction. The tumor number in intestinal tissues was counted, and the histopathological analysis was performed on colon tissues. Enzyme-linked immunosorbent assay and real-time quantitative polymerase chain reaction were performed to measure relevant protein levels in colon tissues.

Results: LR-AFY06 treatment alleviated weight loss, increased organ index, reduced intestinal tumor incidence, improved histopathological damage, decreased the levels of inflammatory cytokines such as interleukin-6 (IL-6), interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), nuclear factor κB (NF-κB), and inducible nitric oxide synthase (iNOS) in the serum and colon tissue, downregulated the mRNA expression of inhibitor of NF-κB beta (IκBβ), p65, p50, p52, B-cell lymphoma-2 (Bcl-2), and B-cell lymphoma-extra large (Bcl-xL) in colon tissues, and increased the mRNA expression of Bid and caspase-8. The high concentration of LR-AFY06 exerted a better effect than the low concentration; however, the effect was slightly inferior to that of aspirin. Moreover, LR-AFY06 mitigated the intestinal inflammatory process and inhibited intestinal tumor development by regulating the NF-κB and apoptosis pathways.

Discussion: The present study indicates the regulatory potential of LR-AFY06 in inflammation-associated colorectal cancer in mice, providing a valuable basis for further research.

Keywords: Lacticaseibacillus rhamnosus; azoxymethane; colon cancer; mRNA; mice.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
The effect of LR-AFY06 on the body weight of mice.
FIGURE 2
FIGURE 2
The effect of LR-AFY06 on colon tissues in mice. (A) Colon length; (B) Visceral index (colon); (C) Colon coefficient; (D) Number of colon tumors. a–cDifferent lowercase letters indicate significant differences (P < 0.05).
FIGURE 3
FIGURE 3
The effect of LR-AFY06 on the histopathology of mouse colon tissues (40×).
FIGURE 4
FIGURE 4
The effect of LR-AFY06 on the serum levels of inflammatory cytokines in mice. (A) IL-1β; (B) IL-6; (C) TNF-α; (D) NF-κB; (E) iNOS. a–dDifferent lowercase letters indicate significant differences (P < 0.05).
FIGURE 5
FIGURE 5
The effect of LR-AFY06 on the colon tissue levels of inflammatory cytokines in mice. (A) IL-1β; (B) IL-6; (C) TNF-α; (D) NF-κB; (E) iNOS. a–dDifferent lowercase letters indicate significant differences (P < 0.05).
FIGURE 6
FIGURE 6
The effect of LR-AFY06 on the relative mRNA expressions of inflammatory factors in mouse colon tissues. (A) Relative expression of IκBβ; (B) Relative expression of p65; (C) Relative expression of p50; (D) Relative expression of p52. a–dDifferent lowercase letters indicate significant differences (P < 0.05).
FIGURE 7
FIGURE 7
The effect of LR-AFY06 on the relative mRNA expressions of apoptosis-related factors in mouse colon tissues. (A) Relative expression of Bid; (B) Relative expression of Bcl-2; (C) Relative expression of Bcl-xL; (D) Relative expression of caspase-8. a–dDifferent lowercase letters indicate significant differences (P < 0.05).
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