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Comparative Study
. 1985 Apr;82(8):2267-71.
doi: 10.1073/pnas.82.8.2267.

Purification and characterization of two cartilage-inducing factors from bovine demineralized bone

S M Seyedin, T C Thomas, A Y Thompson, D M Rosen, K A Piez
Comparative Study

Purification and characterization of two cartilage-inducing factors from bovine demineralized bone

S M Seyedin et al. Proc Natl Acad Sci U S A. 1985 Apr.

Abstract

Two naturally occurring peptides that induce chondrogenesis in culture have been purified to apparent homogeneity. These cartilage-inducing factors (CIF-A and CIF-B) were isolated from bovine demineralized bone by dissociative extraction, gel filtration, cation-exchange chromatography, and reversed-phase HPLC. CIF-A and CIF-B at concentrations of 1-10 ng/ml each induce embryonic rat mesenchymal cells in culture to assume a cartilage morphology and synthesize cartilage-specific proteoglycan and type II collagen. The amino acid compositions of CIF-A and CIF-B are similar but not identical. Both factors have an apparent Mr of 26,000, as determined by NaDodSO4/PAGE. In the presence of 2-mercaptoethanol, both are converted to species of about one-half that Mr, indicating that they are dimers of identical or very similar chains.

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References

    1. Science. 1965 Nov 12;150(3698):893-9 - PubMed
    1. J Bone Joint Surg Am. 1968 Mar;50(2):311-25 - PubMed
    1. Arch Biochem Biophys. 1969 Mar;130(1):337-46 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Biochemistry. 1971 Apr 27;10(9):1652-9 - PubMed

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