Draft genome sequence data of multidrug-resistant Pseudomonas aeruginosa WO7 from a hospital wastewater treatment plant in Thailand

Abstract

Multidrug-resistant Pseudomonas aeruginosa WO7 was isolated from an untreated water sample from a hospital wastewater treatment plant in Thailand. This report presents the draft genome sequence data of P. aeruginosa WO7. Genomic DNA was obtained from a pure culture of P. aeruginosa WO7, and paired-end reads were generated using an Illumina MiSeq sequencer. The draft genome consisted of 111 contigs with a total size of 6,784,206 base pairs, an N50 of 209,424 base pairs, and a GC content of 65.85%. The dDDH value between WO7 and Pseudomonas aeruginosa DSM 50071^T was determined to be 90.7%, indicating that the strain is Pseudomonas aeruginosa. The data presented indicate the potential for bacterial classification, comparative genomics, comprehensive analysis of antimicrobial resistance, and assessment of bacterial virulence factors in P. aeruginosa. The draft genome sequence data have been deposited at the NCBI under Bioproject accession number PRJNA550309.

Keywords: Fluoroquinolone; Fosfomycin; Multidrug resistance; NDM-1; OXA-10; OXA-50; Sulphonamide; VEB-1.

Fig. 1

The genome map of P. aeruginosa WO7 was generated using Proksee . The coding DNA sequences (CDSs) are indicated by blue arrows, and the contigs are represented by grey arrows. GC skew+ and GC skew- are represented by green and purple peaks, respectively, and GC content is indicated by black peaks.

Fig. 2

The phylogenomic tree was reconstructed using whole-genome sequence data from P. aeruginosa WO7 and its closely related type strain on the TYGS platform. Branch numbers were determined on the basis of pseudo-bootstrap support values greater than 80% from 100 replicates using Genome Blast Distance Phylogeny (GBDP), with an average branch support of 95.7%.