Rapid processing of the carboxyl terminus of a trypanosome variant surface glycoprotein
- PMID: 3858818
- PMCID: PMC397744
- DOI: 10.1073/pnas.82.10.3207
Rapid processing of the carboxyl terminus of a trypanosome variant surface glycoprotein
Abstract
The variant surface glycoprotein of the parasite Trypanosoma brucei contains a glycolipid of unknown structure covalently attached to its COOH terminus. We have shown, by using metabolic labeling with [35S]methionine or [3H]myristic acid, precipitation with specific antibodies, and NaDodSO4/polyacrylamide gel electrophoresis, that this glycolipid is attached to the variant surface glycoprotein polypeptide within 1 min after its translation.
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