Evaluation of Molecular Epidemiology of IAPV in Several Regions of Iran

Abstract

In this study, the Israeli acute paralysis virus (IAPV), a single-stranded RNA virus, was investigated in honey bee colonies, which had a history of mortality, population decline, and parasitic diseases. Samples (adult honey bees) were collected from 328 apiaries from three provinces (Tehran, Alborz, and Mazandaran) of Iran to detect IAPV. After sample preparation, RNA was extracted and cDNA was synthesized to perform the reverse transcription polymerase chain reaction (RT-PCR) method using a PCR primer pair, and a 185 bp fragment was amplified. The results showed that out of 328 samples, 103 (31.4%) samples were positive, which were from Mazandaran (14.33%), Tehran (8.84%), and Alborz (8.23%) provinces. Subsequently, some of the positive samples were sequenced and a phylogenetic tree was drawn. The phylogenetic tree showed that the virus isolates were divided into two distinct groups, including one group that had a high similarity to the European acute bee paralysis virus (ABPV) and one group that had a high similarity to the Kashmir bee virus. In addition, the sequences of the samples in three regions were separated in a node from the strains of ABPV from Eastern Europe. Since the length of the branch between the Iranian sequences and the different strains of ABPV from Eastern Europe was short, it can be assumed that the sequences from Iran have a common ancestor with the mentioned strains of ABPV from Eastern Europe.

Keywords: Honey bee; IAPV; Phylogenetic tree; RT-PCR.

Figure 1

The PCR products of 185 bp fragment of standard IAPV as positive control, negative control and collected unknown samples from 3 provinces on 1% agarose gel. Samples 3,7,8 and 10 were positive

Figure 2

The amino acid differences of the research and other similar honey bee pathogenic viruses

Figure 3

Phylogenetic tree and evolutionary relationships of nucleotide sequences of 185 bp fragment of IAPV