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. 2024 Mar 31:53:101388.
doi: 10.1016/j.gore.2024.101388. eCollection 2024 Jun.

Association between serum P16ink4A concentration and CIN and cervical cancer among women attending a cervical cancer clinic in western Uganda: A case control study

Frank Ssedyabane  1 , Joseph Ngonzi  2 , Deusdedit Tusubira  3 , Josephine Nambi Najjuma  4 , Rogers Kajabwangu  2 , Christopher Okeny  1 , Doreen Nuwashaba  1 , Alexcer Namuli  2 , Nixon Niyonzima  5
Affiliations

Association between serum P16ink4A concentration and CIN and cervical cancer among women attending a cervical cancer clinic in western Uganda: A case control study

Frank Ssedyabane et al. Gynecol Oncol Rep. .

Abstract

Introduction: Tissue expression of P16ink4A is correlated with cervical lesions. In this study we determined the association between serum P16ink4A concentrations and cervical lesions among women attending the cervical cancer clinic at Mbarara Regional Hospital (MRRH) South Western Uganda.

Material and methods: We recruited 90 cervical intraepithelial neoplasia (CIN) cases, 90 cervical cancer (CC) cases before treatment and 90 controls. Clinical and demographic data were recorded. Serum P16ink4A concentrations were measured by quantitative Elisa. Cases were confirmed with cytology and/or histology. Descriptive statistics and logistic regression were done with STATA 17 and P-values of <0.05 were considered statistically significant.

Results: The mean serum P16ink4A concentration among CIN cases, CC cases and controls was 1.11(+/-0.66) ng/ml, 1.45(+/-1.11) ng/ml and 1.13(+/-0.61) ng/ml respectively (p = 0.008). 50 % of CIN cases and controls as well as 60 % of CC cases had P16ink4A concentration above 0.946 ng/ml. There were increased odds of CIN for serum P16ink4A though statistically insignificant (AOR: 1.11, p-value: 0.70). There was also a statistically significant reduction in odds of CC for serum P16ink4A (AOR: 0.55, p-value: 0.01).

Conclusion: Serum P16ink4A may likely be associated with cervical lesions especially CC in our study population and this may aid detection of such lesions. Diagnostic utility studies for circulating P16ink4A in detection of cervical cancer are recommended.

Keywords: Cervical cancer; Cervical intraepithelial neoplasia; Mbarara Uganda; P16ink4A; Serum.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Photomicrographs. A-Pap smear negative of intraepithelial lesion or malignancy ×400; B- Pap Smear Atypical squamous cells of undetermined significance ×400; C- Pap Smear Low grade squamous intraepithelial lesion ×400; D- Benign cervical tissue (Haematoxylin and Eosin) ×400; E- Squamous cell carcinoma (Haematoxylin and Eosin) ×400. Fig. 1: A box plot showing serum concentration of P16ink4A across different groups of study participants. CC: Cervical Cancer; CIN: Cervical Intraepithelial Neoplasia. Fig. 1: A-Pap smear negative of intraepithelial lesion or malignancy ×400; B- Pap Smear Atypical squamous cells of undetermined significance ×400; C- Pap Smear Low grade squamous intraepithelial lesion ×400; D- Benign cervical tissue (Haematoxylin and Eosin) ×400; E- Squamous cell carcinoma (Haematoxylin and Eosin) ×400.
Fig. 2
Fig. 2
A box plot showing serum concentration of P16ink4A across different groups of study participants. CC: Cervical Cancer; CIN: Cervical Intraepithelial Neoplasia.
See this image and copyright information in PMC

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