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. 2024 Feb 28;9(1):87-97.
doi: 10.5194/jbji-9-87-2024. eCollection 2024.

Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study

Stéphanie Pascual  1 , Brooklyn Noble  2 , Nusreen Ahmad-Saeed  3 , Catherine Aldridge  4 , Simone Ambretti  5 , Sharon Amit  6 , Rachel Annett  7 , Shaan Ashk O'Shea  3 , Anna Maria Barbui  8 , Gavin Barlow  9 , Lucinda Barrett  10 , Mario Berth  11 , Alessandro Bondi  12 , Nicola Boran  13 , Sara E Boyd  14 , Catarina Chaves  15 , Martin Clauss  16 , Peter Davies  17 , Ileana T Dianzo-Delgado  18 , Jaime Esteban  19 , Stefan Fuchs  20 , Lennart Friis-Hansen  21   22 , Daniel Goldenberger  16 , Andrej Golle  23 , Juha O Groonroos  24 , Ines Hoffmann  25 , Tomer Hoffmann  6 , Harriet Hughes  7 , Marina Ivanova  26 , Peter Jezek  27 , Gwennan Jones  7 , Zeynep Ceren Karahan  28 , Cornelia Lass-Flörl  20 , Frédéric Laurent  29 , Laura Leach  10 , Matilde Lee Horsbøll Pedersen  21   22 , Caroline Loiez  30 , Maureen Lynch  13 , Robert J Maloney  9 , Martin Marsh  4 , Olivia Milburn  4 , Shanine Mitchell  7 , Luke S P Moore  14 , Lynn Moffat  17 , Marianna Murdjeva  31 , Michael E Murphy  17 , Deepa Nayar  4 , Giacomo Nigrisoli  5 , Fionnuala O'Sullivan  13 , Büşra Öz  28 , Teresa Peach  7 , Christina Petridou  32 , Mojgan Prinz  33 , Mitja Rak  34 , Niamh Reidy  13 , Gian Maria Rossolini  35 , Anne-Laure Roux  36 , Patricia Ruiz-Garbajosa  37 , Kordo Saeed  3 , Llanos Salar-Vidal  19 , Carlos Salas Venero  38 , Mathyruban Selvaratnam  10 , Eric Senneville  30 , Peter Starzengruber  39 , Ben Talbot  17 , Vanessa Taylor  7 , Rihard Trebše  34 , Deborah Wearmouth  9 , Birgit Willinger  39 , Marjan Wouthuyzen-Bakker  40 , Brianne Couturier  2 , Florence Allantaz  1
Affiliations

Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study

Stéphanie Pascual et al. J Bone Jt Infect. .

Abstract

Introduction: The BIOFIRE Joint Infection (JI) Panel is a diagnostic tool that uses multiplex-PCR testing to detect microorganisms in synovial fluid specimens from patients suspected of having septic arthritis (SA) on native joints or prosthetic joint infections (PJIs). Methods: A study was conducted across 34 clinical sites in 19 European and Middle Eastern countries from March 2021 to June 2022 to assess the effectiveness of the BIOFIRE JI Panel. Results: A total of 1527 samples were collected from patients suspected of SA or PJI, with an overall agreement of 88.4 % and 85 % respectively between the JI Panel and synovial fluid cultures (SFCs). The JI Panel detected more positive samples and microorganisms than SFC, with a notable difference on Staphylococcus aureus, Streptococcus species, Enterococcus faecalis, Kingella kingae, Neisseria gonorrhoeae, and anaerobic bacteria. The study found that the BIOFIRE JI Panel has a high utility in the real-world clinical setting for suspected SA and PJI, providing diagnostic results in approximately 1 h. The user experience was positive, implying a potential benefit of rapidity of results' turnover in optimising patient management strategies. Conclusion: The study suggests that the BIOFIRE JI Panel could potentially optimise patient management and antimicrobial therapy, thus highlighting its importance in the clinical setting.

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Conflict of interest statement

At least one of the (co-)authors is a member of the editorial board of Journal of Bone and Joint Infection. The peer-review process was guided by an independent editor, and the authors also have no other competing interests to declare.

Figures

Figure 1
Figure 1
Distribution of microorganisms in native joint infections (on-panel).
Figure 2
Figure 2
Distribution of microorganisms in prosthetic joint infections (on-panel).
See this image and copyright information in PMC

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