Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 May 10:132:111985.
doi: 10.1016/j.intimp.2024.111985. Epub 2024 Apr 10.

β-glucan mitigates ovalbumin-induced airway inflammation by preventing oxidative stress and CD8+ T cell infiltration

Islam Ahmed Abdelmawgood  1 , Mohamed A Kotb  2 , Hamid Ashry  3 , Bassam W Ebeed  2 , Noha A Mahana  2 , Ayman Saber Mohamed  2 , Jehane I Eid  2 , Marwa A Ramadan  4 , Nahla S Rabie  2 , Mariam Y Mohamed  2 , Nermeen Th Saed  2 , Nada Yasser  2 , Dina Essam  2 , Youssef Y Zaki  2 , Samar Saeed  2 , Asmaa Mahmoud  2 , Marwan M Eladawy  2 , Abeer Mahmoud Badr  2
Affiliations

β-glucan mitigates ovalbumin-induced airway inflammation by preventing oxidative stress and CD8+ T cell infiltration

Islam Ahmed Abdelmawgood et al. Int Immunopharmacol. .

Abstract

Background: Bronchial asthma is a severe respiratory condition characterized by airway inflammation, remodeling, and oxidative stress. β-Glucan (BG) is a polysaccharide found in fungal cell walls with powerful immunomodulatory properties. This study examined and clarified the mechanisms behind BG's ameliorativeactivitiesin an allergic asthma animal model.

Method: BG was extracted from Chaga mushroom and characterized using FT-IR, UV-visible, zeta potential, and 1H NMR analysis. The mice were divided into five groups, including control, untreated asthmatic, dexamethasone (Dexa)-treated (1 mg/kg), and BG (30 and 100 mg/kg)-treated groups.

Results: BG treatment reduced nasal scratching behavior, airway-infiltrating inflammatory cells, and serum levels of IgE significantly. Additionally, BG attenuated oxidative stress biomarkers by lowering malonaldehyde (MDA) concentrations and increasing the levels of reduced glutathione (GSH), glutathione peroxidase (GPx), and catalase (CAT). Immunohistochemical and flow cytometric analyses have confirmed the suppressive effect of BG on the percentage of airway-infiltrating cytotoxic CD8+ T cells.

Conclusion: The findings revealed the role of CD8+ T cells in the pathogenesis of asthma and the role of BG as a potential therapeutic agent for asthma management through the suppression of airway inflammation and oxidative stress.

Keywords: Allergic asthma; Beta-glucan; CD8(+) T cells; Inflammation; Ovalbumin; Oxidative stress.

PubMed Disclaimer

Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

MeSH terms