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. 2020 Mar;1(1):e4.
doi: 10.1002/viw2.4. Epub 2020 Mar 23.

Promising methods for detection of novel coronavirus SARS-CoV-2

Ran Liu  1 , Aisi Fu  1   2 , Zixin Deng  1   3   4 , Yan Li  5 , Tiangang Liu  1   4
Affiliations

Promising methods for detection of novel coronavirus SARS-CoV-2

Ran Liu et al. View (Beijing). 2020 Mar.

Abstract

A very recent outbreak of the novel coronavirus, COVID-19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection. In this report, we summarized the six promising methods, including whole-genome sequencing, real-time reverse transcription polymerase chain reaction, nanopore target sequencing, antibody-based immunoassay techniques, use of paper-based biomolecular sensors, and the clustered regularly interspaced short palindromic repeats-Cas system-based technology, which can also be deployed for the detection of SARS-CoV-2. We further introduced the principles of these methods, discussed the scope and practicability of application of the available products and methods, and highlighted the potential approaches to develop additional products and techniques for early diagnosis of COVID-19.

Keywords: 2019‐nCoV; COVID‐19; SARS‐CoV‐2; coronavirus; early diagnosis; nucleic acid detection.

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Conflict of interest statement

There is no conflict of interest to declare.

Figures

FIGURE 1
FIGURE 1
Developed and promising methods for detection of novel coronavirus SARS‐CoV‐2
See this image and copyright information in PMC

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