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. 2024 May;10(3):e1447.
doi: 10.1002/vms3.1447.

Establishment of the HPLC fluorescence detection method for plasma trace ivermectin and its pharmacokinetics in Bactrian camel

Bin Hou  1 , Haifeng Wang  2 , Nan Jiang  1 , Bilige Haosi  3 , Surong Hasi  1
Affiliations

Establishment of the HPLC fluorescence detection method for plasma trace ivermectin and its pharmacokinetics in Bactrian camel

Bin Hou et al. Vet Med Sci. 2024 May.

Abstract

Background and objective: Ivermectin (IVM), a widely used veterinary anthelmintic, lacks recommended doses for Bactrian camels. This study aims to establish its pharmacokinetics in Bactrian camels, comparing with other livestock.

Methods: A method for high-performance liquid chromatography fluorescence detection of IVM in plasma was developed.

Results: IVM exhibited linear scaling (y = 0.6946x + 0.0088, R2 = 0.9988) within 0.025-5 ng/mL, with a lower limit of quantification of 25.00 pg/mL, high recovery (>70%) and low RSD (<7%). In Bactrian camels, IVM injection showed a low Cmax, extended Tmax and apparent secondary absorption compared to cattle and sheep.

Conclusions: Slow absorption and widespread distribution were observed, with peak concentration and area under the curve correlating positively with the dose. This study provides insights into IVM pharmacokinetics in Bactrian camels, informing dose determination and highlighting potential metabolic differences compared to other livestock.

Keywords: Bactrian camel; HPLC fluorescence detection; ivermectin injection; pharmacokinetic characteristics.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.

Figures

FIGURE 1
FIGURE 1
Chromatogram of control plasma.
FIGURE 2
FIGURE 2
Chromatogram of doramectin (DOR) and ivermectin (IVM) standards.
FIGURE 3
FIGURE 3
Chromatogram of the Bactrian camel plasma samples.
FIGURE 4
FIGURE 4
Standard curve diagram of ivermectin (IVM) concentration‐IVM peak area/doramectin (DOR) peak area.
FIGURE 5
FIGURE 5
Mean (n = 6) plasma concentrations of ivermectin (IVM) low‐dose group (0.2 mg/kg) after subcutaneous administration to Bactrian camel.
FIGURE 6
FIGURE 6
Mean (n = 6) plasma concentrations of ivermectin (IVM) high‐dose group (0.3 mg/kg) after subcutaneous administration to Bactrian camel.
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References

    1. Al Anazi, A. D. , & Alyousif, M S. (2011). Prevalence of non‐strongyle gastrointestinal parasites of horses in Riyadh region of Saudi Arabia. Saudi Journal of Biological Sciences, 18(3), 299–303. - PMC - PubMed
    1. Ali, B. H. , Oukessou, M. , & Bashir, A. K. (1996). Pharmacokinetic considerations in the camel (Camelus dromedarius): A review. Veterinary Biochemistry and Physiology, 115(1), 1–9. - PubMed
    1. Armour, J. , Bairden, K. , & Preston, J. M. (1980). Anthelmintic efficiency of IVM against naturally acquired bovine gastrointestinal nematodes. The Veterinary Record, 107, 226–227. - PubMed
    1. Badri, M. , Oukessou, M. , Sutra, J. E. , Galrier, P. , & Alvinerie, M. (1996). Pharmacokinetics of IVM in the camel (Camelus dromedarius). Veterinary Result, 11(1), 5–9. - PubMed
    1. Ballweber, L. R. (2009). Ecto‐ and endoparasites of new world camelids. Veterinary Clinics of North America: Food Animal Practice, 25(2), 295–310. - PubMed

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