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. 2024 Jul;36(4):506-509.
doi: 10.1177/10406387241245948. Epub 2024 Apr 14.

Validation of a commercial ELISA kit to measure 11-oxoetiocholanolone in equine and bovine feces

Aloma Zoratti  1 , Isabella Pividori  1 , Antonella Comin  1 , Alberto Prandi  1 , Tanja Peric  1
Affiliations

Validation of a commercial ELISA kit to measure 11-oxoetiocholanolone in equine and bovine feces

Aloma Zoratti et al. J Vet Diagn Invest. 2024 Jul.

Abstract

Feces are a noninvasive and easily collectible matrix that may help determine cumulative hormone metabolite concentrations over medium-to-long times. To date, 11-oxoetiocholanolone, an important metabolite of cortisol, has been measured in equine and bovine feces solely by an in-house enzyme immunoassay (EIA). Therefore, we validated the use of a commercial ELISA kit (11-oxoetiocholanolone ELISA kit; Cayman Chemical), which had been validated on sheep feces and human urine, to measure 11-oxoetiocholanolone in feces from 42 horses and 32 bulls. The ELISA kit had good precision (intra- and inter-assay CVs: 5.8% and 11.2% for equine feces; 9.9% and 11.2% for bovine feces, respectively), analytical sensitivity (0.186 ng/mL for both equine and bovine feces), and accuracy (parallelism and recovery tests) in determining 11-oxoetiocholanolone concentrations in feces from both species. We found ranges of 11-oxoetiocholanolone concentrations of 1-109 ng/g in equine feces and 40-302 ng/g in feces of bulls. The Cayman Chemical ELISA kit offers a simple and accessible means of analysis of 11-oxoetiocholanolone in equine and bovine fecal samples.

Keywords: 11-ketoetiocholanolone; cattle; feces; horses; method validation.

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Conflict of interest statement

Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
Graphical plot for the parallelism obtained with a commercial ELISA kit for 11-oxoetiocholanolone (Cayman Chemical) on equine feces. The relationship between equine fecal 11-oxoetiocholanolone concentrations and the standard 11-oxoetiocholanolone curves was given by the equation y = 1.074x – 0.094.
Figure 2.
Figure 2.
Graphical plot for the parallelism obtained with a commercial ELISA kit for 11-oxoetiocholanolone (Cayman Chemical) on bovine feces. The relationship between bovine fecal 11-oxoetiocholanolone concentrations and the standard 11-oxoetiocholanolone curves was given by the equation y = 1.091x – 0.076.
See this image and copyright information in PMC

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