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. 1985 Sep;82(17):5641-5.
doi: 10.1073/pnas.82.17.5641.

Molecular cloning of an activated human oncogene, homologous to v-raf, from primary stomach cancer

K Shimizu, Y Nakatsu, M Sekiguchi, K Hokamura, K Tanaka, M Terada, T Sugimura

Molecular cloning of an activated human oncogene, homologous to v-raf, from primary stomach cancer

K Shimizu et al. Proc Natl Acad Sci U S A. 1985 Sep.

Abstract

Transfection with high molecular weight DNA from a primary stomach cancer induced foci of transformed NIH 3T3 cells, and the transformed cells were tumorigenic in nude mice. By screening with a human Alu-family probe, we isolated the human DNA sequence from the secondary transformant cells. This transforming sequence encompasses about 60 kilobase pairs and is unrelated to known human transforming genes. Examination of homologies between this sequence and retroviral oncogenes revealed that the human transforming sequence is closely related to the v-raf oncogene of murine transforming retrovirus 3611-MSV.

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References

    1. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4218-22 - PubMed
    1. Br J Cancer. 1960 Mar;14:77-82 - PubMed
    1. Cell. 1979 Apr;16(4):777-85 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3637-40 - PubMed
    1. J Mol Biol. 1960 Dec;2:392-415 - PubMed

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