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. 1985 Sep;82(17):5846-9.
doi: 10.1073/pnas.82.17.5846.

Microdissection and microcloning of the mouse X chromosome

E M Fisher, J S Cavanna, S D Brown

Microdissection and microcloning of the mouse X chromosome

E M Fisher et al. Proc Natl Acad Sci U S A. 1985 Sep.

Abstract

A wild mouse (CD) karyotype in which all the chromosomes bar the X, 19, and Y, are fused as metacentrics has been used for the microdissection and microcloning of a specific mouse X chromosome region. Dissection of a proximal region of the X chromosome encompassing the genetic loci Hprt to Tfm and including mdx has yielded 650 clones. A number of the recovered clones containing sizable inserts have been confirmed as X chromosome specific. This X chromosome bank of clones provides a start point for the isolation of the mdx locus. It is now clear that microdissection and microcloning can be applied to all mouse chromosomes, including the X chromosome, yielding premapped banks of clones that will greatly aid in the isolation and characterization of important genetic loci.

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