Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Sep;82(17):5846-9.
doi: 10.1073/pnas.82.17.5846.

Microdissection and microcloning of the mouse X chromosome

E M Fisher, J S Cavanna, S D Brown

Microdissection and microcloning of the mouse X chromosome

E M Fisher et al. Proc Natl Acad Sci U S A. 1985 Sep.

Abstract

A wild mouse (CD) karyotype in which all the chromosomes bar the X, 19, and Y, are fused as metacentrics has been used for the microdissection and microcloning of a specific mouse X chromosome region. Dissection of a proximal region of the X chromosome encompassing the genetic loci Hprt to Tfm and including mdx has yielded 650 clones. A number of the recovered clones containing sizable inserts have been confirmed as X chromosome specific. This X chromosome bank of clones provides a start point for the isolation of the mdx locus. It is now clear that microdissection and microcloning can be applied to all mouse chromosomes, including the X chromosome, yielding premapped banks of clones that will greatly aid in the isolation and characterization of important genetic loci.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc R Soc Lond B Biol Sci. 1974 Nov 5;187(1088):243-68 - PubMed
    1. Virology. 1973 Sep;55(1):183-92 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3138-42 - PubMed
    1. Lab Anim. 1979 Oct;13(4):301-4 - PubMed
    1. Nucleic Acids Res. 1980 Feb 25;8(4):781-92 - PubMed

Publication types

LinkOut - more resources