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. 2024 Jun 5;68(6):e0013324.
doi: 10.1128/aac.00133-24. Epub 2024 Apr 16.

Genomic characterization of ST38 NDM-5-producing Escherichia coli isolates from an outbreak in the Czech Republic

Katerina Chudejova  1 , Tsolaire Sourenian  1 , Jana Palkovicova  2 , Katarina Stredanska  2 , Lucie Nechutna  1 , Katerina Vlkova  1 , Vendula Studentova  1 ; Working Group for Monitoring of Antibiotic Resistance; Jaroslav Hrabak  1 , Costas C Papagiannitsis  3 , Monika Dolejska  1   2   4   5 , Ibrahim Bitar  1 ; Working group for monitoring of antibiotic resistance
Collaborators, Affiliations

Genomic characterization of ST38 NDM-5-producing Escherichia coli isolates from an outbreak in the Czech Republic

Katerina Chudejova et al. Antimicrob Agents Chemother. .

Abstract

A 2-year national genomic screening in the Czech Republic identified a notable prevalence of the New Delhi metallo-β-lactamase 5 (NDM-5)-producing Escherichia coli sequence type 38 (ST38) in the city of Brno. Forty-two ST38 E. coli isolates harbored the blaNDM-5 gene on the chromosome. Virulence factors confirmed the persistence of these isolates through biofilm formation. Single Nucleotide Polymorphisms (SNPs)-based phylogeny and CRISPR assay typing showed minimal genomic variations, implying a clonally driven outbreak. Results suggest that this high-risk clone may impose a nationwide problem.

Keywords: Escherichia coli; ST38; blaNDM-5.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
The 2D map of Brno and adjacent regions showing the number of detected isolates identified as NDM-5-producing ST38 E. coli strains. Dark red points correspond to 26 isolates, light red to 9, dark pink to 3, and light pink to 1.
Fig 2
Fig 2
The phylogenetic analysis of the clade harboring Czech isolates. The Czech isolates node is colored in red. The outer membrane ring corresponds to the isolate’s reporting country.
See this image and copyright information in PMC

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