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. 2020;5(2):205-209.
doi: 10.1007/s41403-020-00103-z. Epub 2020 May 25.

Label-Free Electrochemical Detection of DNA Hybridization: A Method for COVID-19 Diagnosis

Suryasnata Tripathy  1 , Shiv Govind Singh  1
Affiliations

Label-Free Electrochemical Detection of DNA Hybridization: A Method for COVID-19 Diagnosis

Suryasnata Tripathy et al. Trans Indian Natl Acad Eng. 2020.

Abstract

This paper presents label-free electrochemical transduction as a suitable scheme for COVID-19-specific viral RNA/c-DNA detection, with an aim to facilitate point of care diagnosis. In lieu of this, we discuss the proposed electrochemical biosensing scheme, based on electrodeposited gold nanoparticles as the transducing elements. Specific to this approach, here, the protocols associated with the immobilization of the single-stranded probe nucleotide on to the biosensor, have also been laid out. This paper also discusses the methods of electrochemical analysis, to be used for data acquisition and subsequent calibration, in relation to target analyte detection. Towards facilitating portable diagnosis, development of miniaturized sensors and their integration with readout units have also been discussed.

Keywords: COVID-19; Electrochemical Sensors; Miniaturization.

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Conflict of interest statement

Conflict of interestThere is no conflict of interest.

Figures

Fig. 1
Fig. 1
A schematic representation of the miniaturized electrochemical platform showing different components, with actual image of two fabricated devices
Fig. 2
Fig. 2
A schematic representation of the probe DNA immobilization protocol on to the gold sensing electrodes, and the hybridization of the target nucleotide
Fig. 3
Fig. 3
A schematic representation of the envisaged interfacing of the device with a smartphone
See this image and copyright information in PMC

References

    1. Akhavan O, Ghaderi E, Rahighi R (2012) Toward single-DNA electrochemical biosensing by graphene nanowalls. ACS Nano 6(4):2904–2916 - PubMed
    1. Alwine JC, Kemp DJ, Stark GR (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci 74(12):5350–5354 - PMC - PubMed
    1. Bakker E, Telting-Diaz M (2002) Electrochemical sensors. Anal Chem 74(12):2781–2800 - PubMed
    1. Boon EM, Ceres DM, Drummond TG, Hill MG, Barton JK (2000) Mutation detection by electrocatalysis at DNA-modified electrodes. Nat Biotechnol 18(10):1096–1100 - PubMed
    1. Drummond TG, Hill MG, Barton JK (2003) Electrochemical DNA sensors. Nat Biotechnol 21(10):1192 - PubMed

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