Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Aug 10;6(11):3651-60.
doi: 10.1093/nar/6.11.3651.

Isolation of a single polypeptide leucyl-tRNA synthetase from bakers' yeast

C S Lin, R Irwin, J G Chirikjian
Free PMC article

Isolation of a single polypeptide leucyl-tRNA synthetase from bakers' yeast

C S Lin et al. Nucleic Acids Res. .
Free PMC article

Abstract

A single polypeptide of leucyl-tRNA synthetase (LRS) has been purified from budding bakers' yeast by a modification of the procedure published earlier. On denaturing polyacrylamide gel electrophoresis LRS was one band corresponding to molecular weight of 120,000 +/- 5,000 daltons. Variable amounts of LRS with a similar molecular weight but which dissociated into equal subunits of 58,000 were also isolated. The affinities (KM) for substrates for this form of the enzyme were similar to those previously reported for the dimeric form of the enzyme.

PubMed Disclaimer

References

    1. Ann N Y Acad Sci. 1964 Dec 28;121:404-27 - PubMed
    1. J Biol Chem. 1961 May;236:1372-9 - PubMed
    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Eur J Biochem. 1975 Feb 3;51(1):237-52 - PubMed
    1. J Biol Chem. 1972 May 10;247(9):2931-43 - PubMed

Publication types

MeSH terms

Substances