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. 1979 Sep 11;7(1):179-92.
doi: 10.1093/nar/7.1.179.

Site specific enzymatic cleavage of RNA

H Donis-Keller

Site specific enzymatic cleavage of RNA

H Donis-Keller. Nucleic Acids Res. .

Abstract

The hybridization of a DNA oligonucleotide a specific tetramer or longer) will direct a cleavage by RNase H (EC 3.1.4.34) to a specific site in RNA. The resulting fragments can then be labeled at their 5' or 3' ends, purified, and sequenced directly. This procedure is demonstrated with two RNA molecules of known sequence: 5.8S rRNA from yeast (158 nucleotides) and satellite tobacco necrosis virus (STNV) RNA (1240 nucleotides).

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References

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