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Review
. 2024 Jun;242(5):1911-1918.
doi: 10.1111/nph.19734. Epub 2024 Apr 16.

Isotopically nonstationary metabolic flux analysis of plants: recent progress and future opportunities

Somnath Koley  1 , Poonam Jyoti  1 , Maneesh Lingwan  1 , Doug K Allen  1   2
Affiliations
Free article
Review

Isotopically nonstationary metabolic flux analysis of plants: recent progress and future opportunities

Somnath Koley et al. New Phytol. 2024 Jun.
Free article

Abstract

Metabolic flux analysis (MFA) is a valuable tool for quantifying cellular phenotypes and to guide plant metabolic engineering. By introducing stable isotopic tracers and employing mathematical models, MFA can quantify the rates of metabolic reactions through biochemical pathways. Recent applications of isotopically nonstationary MFA (INST-MFA) to plants have elucidated nonintuitive metabolism in leaves under optimal and stress conditions, described coupled fluxes for fast-growing algae, and produced a synergistic multi-organ flux map that is a first in MFA for any biological system. These insights could not be elucidated through other approaches and show the potential of INST-MFA to correct an oversimplified understanding of plant metabolism.

Keywords: biochemical pathways; carbon partitioning; central carbon metabolism; isotopic labeling; metabolic flux analysis; metabolic modeling; plant metabolism; systems biology.

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References

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    1. Allen DK, Ratcliffe RG. 2009. Quantification of isotope label. In: Schwender J, ed. Plant metabolic networks. Springer New York: New York, NY, USA, 105–149.

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