Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Aug;257(3-4):207-214.
doi: 10.1007/s00232-024-00312-5. Epub 2024 Apr 17.

Abundance of the Membrane Proteome in Yeast Cells Lacking Spc1, a Non-catalytic Subunit of the Signal Peptidase Complex

Chewon Yim #  1 , Yeonji Chung #  1 , Sungjoon Son  1 , Jeesoo Kim  1   2 , Jong-Seo Kim  1   2 , Hyun Kim  3
Affiliations

Abundance of the Membrane Proteome in Yeast Cells Lacking Spc1, a Non-catalytic Subunit of the Signal Peptidase Complex

Chewon Yim et al. J Membr Biol. 2024 Aug.

Abstract

The signal peptidase complex (SPC) mediates processing of signal peptides of secretory precursors. But, recent studies show that the eukaryotic SPC also cleaves internal transmembrane segments of some membrane proteins, and its non-catalytic subunit, Spc1/SPCS1 plays a critical role in this process. To assess the impact of Spc1 on membrane proteostasis, we carried out quantitative proteomics of yeast cells with and without Spc1. Our data show that the abundance of the membrane proteome in yeast cells lacking Spc1 is in general reduced compared to that in wild-type cells, implicating its role in controlling the cellular levels of membrane proteins.

Keywords: Membrane proteins; Quality control; Quantity control; SPCS1; Spc12.

PubMed Disclaimer

References

    1. Almagro Armenteros JJ, Tsirigos KD, Sonderby CK, Petersen TN, Winther O, Brunak S, von Heijne G, Nielsen H (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks. Nat Biotechnol 37:420–423 - DOI - PubMed
    1. Alzahrani N, Wu MJ, Shanmugam S, Yi M (2020) Delayed by design: role of suboptimal signal peptidase processing of viral structural protein precursors in Flaviviridae virus assembly. Viruses 12:1090 - DOI - PubMed - PMC
    1. Avci D, Fuchs S, Schrul B, Fukumori A, Breker M, Frumkin I, Chen CY, Biniossek ML, Kremmer E, Schilling O, Steiner H, Schuldiner M, Lemberg MK (2014) The yeast ER-intramembrane protease Ypf1 refines nutrient sensing by regulating transporter abundance. Mol Cell 56:630–640 - DOI - PubMed
    1. Bachmair A, Finley D, Varshavsky A (1986) In vivo half-life of a protein is a function of its amino-terminal residue. Science 234:179–186 - DOI - PubMed
    1. Berninsone P, Miret JJ, Hirschberg CB (1994) The Golgi guanosine diphosphatase is required for transport of GDP-mannose into the lumen of Saccharomyces cerevisiae Golgi vesicles. J Biol Chem 269:207–211 - DOI - PubMed

MeSH terms

LinkOut - more resources