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. 2024 Jun 1;86(6):653-655.
doi: 10.1292/jvms.24-0037. Epub 2024 Apr 16.

Monoclonal proliferation of B-cells with two integration sites of bovine leukemia virus proviral DNA in cattle with enzootic bovine leukosis

Masaki Maezawa  1 , Shiho Takezawa  2 , Kana Sakaguchi-Maezawa  2 , Yusuke Tanaka  3 , Ken-Ichi Watanabe  2 , Yoshiyasu Kobayashi  2 , Hisashi Inokuma  1   4
Affiliations

Monoclonal proliferation of B-cells with two integration sites of bovine leukemia virus proviral DNA in cattle with enzootic bovine leukosis

Masaki Maezawa et al. J Vet Med Sci. .

Abstract

The present study analyzed B-cell clonality and bovine leukemia virus (BLV) provirus integration sites in cattle with enzootic bovine leukosis (EBL) having BLV proviral copy numbers less or greater than the number of bovine nucleated cells. EBL cattle with BLV copy numbers less than the number of bovine nucleated cells showed monoclonal and biclonal proliferation of B-cells with one BLV provirus integration site. On the other hand, EBL cattle with BLV copy numbers greater than the number of bovine nucleated cells showed monoclonal proliferation of B-cells with two BLV provirus integration sites. These results suggest that superinfection of BLV can occur in EBL cattle.

Keywords: bovine leukemia virus; clonality; enzootic bovine leukosis; superinfection.

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Conflict of interest statement

The Laboratory of OSG Veterinary Science for Global Disease Management is an endowment laboratory, supported by a grant from OSG Corporation.

Figures

Fig. 1.
Fig. 1.
Representative results of B-cell clonality polymerase chain reaction (PCR) and inverse PCR. Peripheral blood from enzootic bovine leukosis (EBL)-20, lymph node from EBL-31, and lymph node from EBL-52 were used. ①: B-cell clonality PCR, ②: inverse PCR, and M: molecular weight marker.
See this image and copyright information in PMC

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References

    1. Aida Y, Murakami H, Takahashi M, Takeshima SN. 2013. Mechanisms of pathogenesis induced by bovine leukemia virus as a model for human T-cell leukemia virus. Front Microbiol 4: 328. doi: 10.3389/fmicb.2013.00328 - DOI - PMC - PubMed
    1. D’Aloja P, Olivetta E, Bona R, Nappi F, Pedacchia D, Pugliese K, Ferrari G, Verani P, Federico M. 1998. gag, vif, and nef genes contribute to the homologous viral interference induced by a nonproducer human immunodeficiency virus type 1 (HIV-1) variant: identification of novel HIV-1-inhibiting viral protein mutants. J Virol 72: 4308–4319. doi: 10.1128/JVI.72.5.4308-4319.1998 - DOI - PMC - PubMed
    1. Hoxie JA, Alpers JD, Rackowski JL, Huebner K, Haggarty BS, Cedarbaum AJ, Reed JC. 1986. Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV. Science 234: 1123–1127. doi: 10.1126/science.3095925 - DOI - PubMed
    1. Kamihira S, Dateki N, Sugahara K, Yamada Y, Tomonaga M, Maeda T, Tahara M. 2000. Real-time polymerase chain reaction for quantification of HTLV-1 proviral load: application for analyzing aberrant integration of the proviral DNA in adult T-cell leukemia. Int J Hematol 72: 79–84. - PubMed
    1. Lama J. 2003. The physiological relevance of CD4 receptor down-modulation during HIV infection. Curr HIV Res 1: 167–184. doi: 10.2174/1570162033485276 - DOI - PubMed