Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Apr 17;114(3):47.
doi: 10.1007/s11103-024-01434-z.

Genome-wide view and characterization of natural antisense transcripts in Cannabis Sativa L

Chang Zhang #  1 , Mei Jiang #  1   2 , Jingting Liu  1 , Bin Wu  3 , Chang Liu  4
Affiliations

Genome-wide view and characterization of natural antisense transcripts in Cannabis Sativa L

Chang Zhang et al. Plant Mol Biol. .

Erratum in

Abstract

Natural Antisense Transcripts (NATs) are a kind of complex regulatory RNAs that play crucial roles in gene expression and regulation. However, the NATs in Cannabis Sativa L., a widely economic and medicinal plant rich in cannabinoids remain unknown. In this study, we comprehensively predicted C. sativa NATs genome-wide using strand-specific RNA sequencing (ssRNA-Seq) data, and validated the expression profiles by strand-specific quantitative reverse transcription PCR (ssRT-qPCR). Consequently, a total of 307 NATs were predicted in C. sativa, including 104 cis- and 203 trans- NATs. Functional enrichment analysis demonstrated the potential involvement of the C. sativa NATs in DNA polymerase activity, RNA-DNA hybrid ribonuclease activity, and nucleic acid binding. Finally, 18 cis- and 376 trans- NAT-ST pairs were predicted to produce 621 cis- and 5,679 trans- small interfering RNA (nat-siRNAs), respectively. These nat-siRNAs were potentially involved in the biosynthesis of cannabinoids and cellulose. All these results will shed light on the regulation of NATs and nat-siRNAs in C. sativa.

Keywords: Cannabis sativa L.; Natural antisense transcripts; nat-siRNAs.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Adal AM, Doshi K, Holbrook L, Mahmoud SS (2021) Comparative RNA-Seq analysis reveals genes associated with masculinization in female Cannabis sativa. Planta 253(1):17
    1. Addo-Quaye C, Miller W, Axtell MJ (2009) CleaveLand: a pipeline for using degradome data to find cleaved small RNA targets. Bioinformatics 25(1):130–131 - PubMed - DOI
    1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25(17):3389–3402 - PubMed - PMC - DOI
    1. Barrell BG, Air GM, Hutchison CA 3rd (1976) Overlapping genes in bacteriophage phiX174. Nature 264(5581):34–41 - PubMed - DOI
    1. Borsani O, Zhu J, Verslues PE, Sunkar R, Zhu JK (2005) Endogenous siRNAs derived from a pair of natural cis-antisense transcripts regulate salt tolerance in Arabidopsis. Cell 123(7):1279–1291 - PubMed - PMC - DOI

LinkOut - more resources