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. 2025 Jan-Feb;101(1):106-115.
doi: 10.1111/php.13952. Epub 2024 Apr 18.

Effect of hydrogen-rich saline on melanopsin after acute blue light-induced retinal damage in rats

Xiao Wang  1   2 , Yifan Sun  1   2 , Changlin Luan  1   2 , Shiqiao Yang  1   2 , Kailei Wang  1   2 , Xiaoran Zhang  1   2 , Rui Hao  1   2   3 , Wei Zhang  1   2   3
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Effect of hydrogen-rich saline on melanopsin after acute blue light-induced retinal damage in rats

Xiao Wang et al. Photochem Photobiol. 2025 Jan-Feb.

Abstract

Excessive exposure to blue light can cause retinal damage. Hydrogen-rich saline (HRS), one of the hydrogen therapies, has been demonstrated to be effective in eye photodamage, but the effect on the expression of melanopsin in intrinsically photosensitive retinal ganglion cells (ipRGCs) is unknown. In this study, we used a rat model of light-induced retinal injury to observe the expression of melanopsin after HRS treatment and to determine the effect of HRS on retinal ganglion cell protection. Adult SD rats were exposed to blue light (48 h) and treated with HRS for 0, 3, 7, and 14 days. Real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB) were performed to find the expression of genes and proteins, respectively. The function of retinal ipRGCs was measured by pattern-evoked electroretinography (pERG). The number and morphological changes of melanopsin-positive ganglion cells in the retina were observed by immunofluorescence (IF). Acute blue light exposure caused a decrease in ipRGC function, decreased expression of melanopsin protein and the melanopsin-positive RGCs, and diminished immunoreactivity in dendrites. However, over time, melanopsin showed a tendency to self-recovery, with an increase in melanopsin protein expression and the number of melanopsin-positive RGCs, with incomplete recovery of function within two weeks. HRS treatment accelerated the recovery process, with a significant increase in melanopsin expression and the number of melanopsin-positive RGCs, and an improvement in the pERG waveform within two weeks.

Keywords: blue light; hydrogen‐rich saline; melanopsin; retinal damage.

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